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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Sumberdaya Hayati
Puji Rianti
Primate Research Center, Bogor Agricultural University, Bogor 16151 Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Diversity and Effectiveness of Insect Pollinators of Jatropha curcas L. (Euphorbiaceae) Puji Rianti; Bambang Suryobroto; Tri Atmowidi
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.679 KB) | DOI: 10.4308/hjb.17.1.38

Abstract

Seed of Jatropha curcas (Euphorbiaceae) is currently established as the source for biofuel Therefore, it is important to understand the diversity insects that pollinated J. curcas inflorescence yellow flowers. We also aimed to study the pollination effects on fruit set on J. curcas. Scan sampling method were carried out to explore the insect pollinators diversity from 07.00 up to 17.00 h  in every 15 minutes. Visiting frequency of pollinators insects were observed by using focal sampling. Those information together with flowering periods, flower nectar volumes, and environmental factors were used as the basic data to determine the effectiveness of insect pollination both in covered and uncovered of seed set plants. Results showed that nine species of insect pollinators were from three order (Hymenoptera, Lepidotera, and Diptera) pollinated J. curcas. Four species of Hymenoptera i.e. Prenolepis, Apis dorsata, Xylocopa confusa, and Apis cerana showed  the highest abundances. The highest abundance and species richness of pollinators occurred at 08.00-10.15 and 15.00-17.15 h. Bees of X. confusa, A. cerana, and A. dorsata of Apidae are effective as insect pollinators in J. curcas plantations, due to high visited frequencies. The insect pollinators also increased fruits and seeds set of J. curcas in the uncovered experiment plants. Thereby, enhancement the three pollinator insects as part of crop management have to be considered by farmers.
Identification of Diagnostic Mitochondrial DNA Single Nucleotide Polymorphisms Specific to Sumatran Orangutan (Pongo abelii) Populations Puji Rianti; Dyah Perwitasari-Farajallah; Dondin Sajuthi; Joko Pamungkas; Alexander Nater; Michael Krützen
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1662.775 KB) | DOI: 10.4308/hjb.22.4.149

Abstract

The hypervariable region I of mitochondrial DNA has frequently been used to distinguish among populations, in particular in species with strong female philopatry. In such cases, populations are expected to diverge rapidly for hypervariable region I markers because of the smaller effective population size and thus increased genetic drift. This rapid divergence leads to the accumulation of mutations exclusively found in one population, which may serve as diagnostic single nucleotide polymorphisms (SNPs). To date, diagnostic SNPs distinctive to Sumatran orangutan populations have not yet been described. However, given the continuously declining numbers of Sumatran orangutans, this information can be vital for effective conservation measures, especially regarding reintroductions of orangutans in rehabilitation centers. Phylogenetic analyses of 54 samples of Sumatran orangutans from nine sampling sites with good provenance, we found five major clades and a total of 20 haplotypes. We propose a total of 52 diagnostic SNPs that are specific to Sumatran orangutan populations. Data can be used to develop restriction fragment length polymorphism assays to carry out genetic assignments using basic laboratory equipment to assign Sumatran orangutan to their population of origin.
Ecology and Conservation of the Endangered Banteng (Bos javanicus) in Indonesia Tropical Lowland Forest Dede Aulia Rahman; Riki Herliansyah; Puji Rianti; Ujang Mamat Rahmat; Asep Yayus Firdaus; Mochamad Syamsudin
HAYATI Journal of Biosciences Vol. 26 No. 2 (2019): April 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.643 KB) | DOI: 10.4308/hjb.26.2.68

Abstract

Banteng, Bos javanicus, as wild cattle is a vital and importance source of germplasm in Indonesia. Various human activities currently threaten their conservation status. Nonetheless, no long-term monitoring programmes are in place for this species. Using distribution point and statistical analysis based on 46,116 camera trap days from December 2015 to January 2017, we aimed to provide habitat preferences, activity patterns and ecological data for banteng population in Ujung Kulon National Park (UKNP). It is the largest population of banteng in Indonesia and is living in a limited habitat area. According to the best occupancy model, the most suitable areas for this species were the secondary forest located at the center portion of UKNP. The presence of the invasive cluster sugar palm, Arenga obtusifolia, in dry season provides additional alternative food for banteng when its main food is scarcer in the forest. Banteng was cathemeral all year round, with the proportion of cathemeral records and the recording rate did not change with the protection of the level area, moon phase or season. To reduce the probability of encountering predators, banteng avoided the space use of dholes. Selection and avoidance of habitats was stronger than avoidance of the predator activity areas. Habitat competition from domestic cattle which grazed illegally in the national park appears to be a problem to the species since zoonosis appears from domestic cattle to banteng. Therefore, effective law enforcement and an adequate conservation strategy are required to eliminate the impacts of both direct and indirect threats.
Application of Environmental DNA (eDNA) Metabarcoding Method to Identify Threatened Sulawesi Mammal Based on 12S rRNA Gene Bambang Suryobroto; Ahmad Abdul Jabbar; Puji Rianti
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.114-121

Abstract

Species detection and identification is a crucial steps in biodiversity assessment. Traditional methods are often invasive and resource intensive. The number of studies demonstrating successful of eDNA metabarcoding approach in species identification has increased rapidly in recent years. Some of large terrestrial mammals have reportedly utilize natural salt licks as a source of minerals in the diet and its genetic material left in the environment can be used to identify species from this site. An eDNA metabarcoding protocol had been carried out to identify Sulawesi mammals from Adudu natural salt-licks, Nantu Wildlife Reserve, Gorontalo. Environmental DNA were extracted from water samples, Amplicon libraries were prepared by PCR amplification and Illumina MiSeq high throughput sequencing. Reads processing and taxonomic assignment carried out in two bioinformatics packages, PipeCraft-1.0 and OBITools-2.11. Two endangered Sulawesi mammals species had been identified, i.e. lowland anoa (Bubalus depressicornis) and babirusa (Babyrousa babyrussa). The accuracy of mammal species identification using eDNA metabarcoding is affected by rigorous experimental procedures, DNA marker reliability, and availability of reference sequence database.
Autentikasi Produk Olahan Ikan Hiu Komersial menggunakan Teknik Species-Specific DNA Mini-barcodes.: Authentication of Commercially Shark Products Using Species-Specific DNA Mini-barcodes Techniques Asadatun Abdullah; Ari Elisa Ratih; Shabrina Aulia; Puji Rianti; Tati Nurhayati; Agoes Mardiono Jacoeb
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 2 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i2.32226

Abstract

Pemanfaatan ikan hiu sebagai bahan baku produk olahan perikanan dapat menyebabkan berkurangnya populasi ikan hiu yang juga rentan akan kepunahan. Ikan hiu pada umumnya diperdagangkan dalam bentuk sirip, daging ikan yang diasinkan dan direbus, minyak ikan serta sebagai bahan subtitusi pada pakan hewan sehingga proses identifikasi menggunakan metode berbasis morfologi dan meristik sulit dilakukan. Sebagai alternatif proses autentikasi bahan baku produk olahan ikan hiu adalah menggunakan teknik DNA mini barcodes. Penelitian ini bertujuan untuk merancang dan mengaplikasikan primer DNA mini barcodes yang spesifik terhadap tiga species hiu terancam punah yang masuk dalam daftar CITES (Sphyrna lewini, Alopias pelagicus, Carcharhinus falciformis) serta mengaplikasikan pada berbagai produk olahan ikan hiu. Tahapan penelitian yang dilakukan meliputi koleksi sampel produk olahan perikanan, perancangan primer spesifik, isolasi DNA, pengujian kualitas dan kuantitas DNA, amplifikasi DNA, dan sekuensing. Berbagai produk olahan komersial berhasil diamplifikasi oleh primer spesifik yaitu pada target 300 pb (S. lewini), 285 pb (A. pelagicus) dan 352 pb (C. falciformes). Hasil sekuensing DNA sampel menunjukkan bahwa spesies teridentifikasi adalah S. lewini, A. pelagicus, C. falciformes dan Hemigaleus microstoma dengan kemiripan 99-100%. Hal tersebut menunjukkan bahwa masih terdapatnya pemanfaatan ikan hiu yang dilindungi secara ilegal, sehingga perlu penerapan regulasi yang lebih ketat untuk melestarikan spesies-spesies hiu yang terancam kepunahannya.
Profil DNA Forensik pada Barang Bukti Dua Kasus Pembunuhan di Indonesia RIANTI, PUJI; CRISTIN, ELISA; WIDODO, PUTUT TJAHJO
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.48-56

Abstract

DNA technology for profiling purposes has been used in many basic and applied science. One of the emerged applied science in genetics is it’s uses in solving crime cases. Homicide became one of highest crime cases in Indonesia. Solving its cases through DNA profiling technology using items of evidence as tool is needed. Here, we report the profiling of human DNA from several items of evidence available in the crime scene and the suspect. We used items of evidence from study cases no. 18098 and 18101, based on the legal permission of Indonesia’s National Police. We used 21 international standards of human STR markers, one sex-determining marker, one Y STR marker, and one independent Y marker to developed human alleles from tissue and blood stains left and/or shred on the victims, soap bottles, knifes, victim’s clothes and ropes as well as the buccal swab of the crime suspect. Our alleles identification matched between the victim and the crime suspect in both cases with the accuracy of DNA profiles compatibility at 99.99%. Detection of DNA profiling is depending on the evidence and time of storage which are influence by environment that can lead to the process of decayed and/or contaminated.
Optimization of Genetic Material Extraction Techniques and Application of Isothermal Amplification Method for Field Authentication of Two Thresher Sharks (Alopias pelagicus and Alopias superciliosus) Aldina, Vatia Dwi Rizki Ramadhani; Abdullah, Asadatun; Rianti, Puji; Jacoeb, Agoes Mardiono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.147-154

Abstract

The pelagic thresher shark (Alopias pelagicus) and bigeye thresher shark (Alopias superciliosus) are important shark species for Indonesia's consumption and finning industry. Both Alopias species are included in the CITES appendix II, thus requiring certain documents for trading. Regarding species identification for on-site application, the DNA isolation method is a crucial step. In this study, we developed a DNA isolation method suitable for on-site application based on isothermal amplification (LAMP) and species-specific COI gene markers. Three different extraction methods were applied, namely modified spin column kits and dipsticks. The quality of DNA was evaluated and tested for isothermal amplification using a reference sample, fresh fillet, and ethanol-preserved sample. The extracted sample concentration was in the range of 135.35-0.65 ng/μL. The LAMP test showed that three different DNA extraction methods successfully amplified the DNA fragments through the color changes at the end point of the LAMP reaction. The LAMP test was also sufficient to detect less than 10 ng of DNA from A. pelagicus and A. superciliosus within 30-50 min. The DNA from the modified spin column and dipstick extraction method combined with LAMP can potentially be used to detect Alopias pelagicus and Alopias superciliosus species on-site.
Relative abundance, activity pattern and habitat suitability of Great Argus (Argusianus argus grayi) in Sungai Wain Protected Forest, Balikpapan, East Kalimantan Ramadhanti, Hanny; Perwitasari-Farajallah, Dyah; Mulyani, Yeni Aryati; Rianti, Puji
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.507-515

Abstract

Great Argus (Argusianus argus) is a globally threatened species that is protected by law in Indonesia. This species is a habitat specialist that tends to be confined to primary forests and is susceptible to human disturbance and environmental change. Sungai Wain Protected Forest (SWPF) is an isolated lowland rainforest in Kalimantan. The rising deforestation rate has threatened species sustainability in recent decades, including the Great Argus in SWPF. This study aimed to systematically analyze camera trap data on the Great Argus's abundance, activity patterns, and habitat suitability prediction in SWPF. We used camera trap data from 2018-2022 at the SWPF. Great Argus's lowest relative abundance index was in 2020, and the highest was in 2022. Overall, the relative abundance index of the Great Argus was approximately 3.125 independent events per 100-day trap night. The activity pattern of the Great Argus started at dawn and then constantly decreased until dusk. The habitat suitability prediction for the Great Argus was mostly in primary forests. Distance to road and building were the variables that contributed the most to the results of habitat suitability modeling. Habitat loss and suitability are the determining factors for the sustainability of significant argus populations.
Impact of Dietary Variation on Gut Microbiota Composition in Macaca fascicularis at the Primate Research Center, IPB University Tambunan, Anggitha; Rianti, Puji; Darusman, Huda Shalahudin; Kyes, Randall C.
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.670-682

Abstract

Long-tailed macaques (Macaca fascicularis: LTMs) are widely distributed, adaptable primates commonly used as animal models in biomedical research. At primate facilities such as the Dramaga Captive Breeding Facility and the Tinjil Island Natural Habitat Breeding Facility at the Primate Research Center, IPB University, LTMs experience varying diets depending on their captive or semi-wild environments. At Dramaga Captive Breeding Facility, LTMs housed in individual and group cages are provided with different feeding regimens, whereas the semi-wild LTMs on Tinjil Island feed primarily on natural food sources with occasional, very modest provisioning of bananas. Our study's objective is to evaluate these diets' influence on the gut microbiota and overall health of LTMs. Through comparative analysis of fecal microbiomes using 16S rRNA sequencing, we identified distinct gut microbiota profiles between captive and semi-wild LTMs. Semi-wild LTMs displayed a predominance of Proteobacteria, notably Succinivibrio, associated with a high-fiber diet derived from wild plant consumption. In contrast, captive LTMs housed in individual and group cages exhibited dominance of Firmicutes and Bacteroidota, respectively. These findings suggest that diet is pivotal in shaping gut bacterial profiles, potentially impacting metabolic functions. Our study provides insight into the relationship between certain diets and gut bacterial composition in LTMs across different breeding facilities at the Primate Research Center, IPB University, thereby offering valuable implications for LTMs' welfare and management strategies.
Crucial Habitats for the Spangled Ebony Langur: The Role of Former Production Forests in Alas Purwo National Park Maulahila, Haikal Idris; Rianti, Puji; Rahman, Dede Aulia
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1485-1506

Abstract

Land use conversion into production forests has led to habitat degradation for primates. On the other hand, most primates have started adapting to the presence of these habitats and have forced conservation efforts to focus on the production forests. Our study at the Kucur Resort, Alas Purwo National Park, reported the population estimates using distance sampling, composition and diversity of vegetation, and habitat suitability of Trachypithecus auratus using multi-algorithm approach in former production forests consisting of jungle and rehabilitation zones. Our study estimated the population density of T. auratus at around 1.29 individuals per km2. We found an association between the number of encounters and the diversity of vegetation in each zone, although it was dominated by Tectona grandis. In addition, we predict that the area of suitable habitat is always smaller than the unsuitable habitat and suggest the model from Boosted Regression Tree as a management reference. We assume that the presence of T. auratus in this habitat is influenced by food availability, distance from settlements, and slope. This study provides reliable information on the potential of reforesting production forests as a habitat for T. auratus and deserve conservation attention.
Co-Authors Agoes Mardiono Jacoeb Ahmad Abdul Jabbar Aldina, Vatia Dwi Rizki Ramadhani Alexander Nater Ari Elisa Ratih Asadatun Abdullah Asep Yayus Firdaus Bambang Suryobroto CRISTIN, ELISA Darusman, Huda Shalahudin DEDE AULIA RAHMAN Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Maulahila, Haikal Idris Michael Krützen Mochamad Syamsudin Pamungkas, Joko Ramadhanti, Hanny Randall C. Kyes Riki Herliansyah Shabrina Aulia Tambunan, Anggitha TATI NURHAYATI Tri Atmowidi Ujang Mamat Rahmat Widodo, Putut Tjahjo Yeni Aryati Mulyani