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All Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Folia Medica Indonesiana Majalah Patologi Indonesia INDRA: Jurnal Pengabdian kepada Masyarakat
Riana Sari Puspita Rasyid
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health Other

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Hubungan Kepadatan Tumor-Associated-Macrophages dan TumorAssociated-Neutrophils dengan Karakteristik Klinikohistopatologi pada Pasien Limfoma Hodgkin Nadya Virana Putri; Neti; Riana Sari Puspita Rasyid; Krisna Murti
Majalah Patologi Indonesia Vol 30 No 2 (2021): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.287 KB) | DOI: 10.55816/mpi.v30i2.471

Abstract

BackgroundThe development and aggressiveness of tumor cells were influenced by tumor microenvironment. Tumor-Associated-Macrophages(TAMs) and Tumor-Associated-Neutrophils (TANs) are components of tumor microenvironment, contribute to development and growthof tumor cells. The aim of this research was to analyse correlation of TAMs and TANs densities with clinicohistopathologiccharacteristics of patients i.e. age, gender, location, and subtype.MethodsA case series was conducted. The samples were paraffin blocks of Hodgkin lymphoma patients at Anatomic Pathology DepartmentFaculty of Medicine Universitas Sriwijaya/RSUP Dr. Mohammad Hoesin Palembang. Among 44 cases of Hodgkin lymphomadiagnosed from 1stJanuari 2015 to 30th October 2019, only 31 cases were included. Immunohistochemistry was performed by usinganti-CD163 antibody and neutrophils were identified by morphologic appearances. The results were correlated to characteristicfactors. Statistical analysis was performed using Spearman and Lambda test with significant consideration if p<0.05.ResultsThe study results showed 30 cases of classical Hodgkin lymphoma (CHL) and 1 case of NLPHL. CHL has only 3 variants LR(lymphocyte-rich) (60%), MC (mixed-cellularity) (26.7%), and NS (nodular sclerosis) (13.3%). Hodgkin lymphoma is most commonlyfound in males (61.3%). Tumor were mostly found in the head and neck, and patient ages were majority in the age group 20-29 years,40-49 years, and over 50 years. Spearman test showed there was a significant correlation between density of TAMs with ages(p=0.043).ConclusionHigh density of TAM was correlated with age, however, there was no correlation between TAN and clinicohistopathologiccharacteristics of Hodgkin lymphoma.
Karakteristik Klinikopatologik serta Hubungan Derajat Keganasan dengan Subtipe Molekular Luminal B pada Pasien Karsinoma Payudara di RSUP Mohammad Hoesin Palembang Tahun 2014-2016 Reihan Putri Awaliah; Citra Dewi; Riana Sari Puspita Rasyid
Majalah Patologi Indonesia Vol 31 No 1 (2022): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.371 KB) | DOI: 10.55816/mpi.v31i1.488

Abstract

BackgroundBreast carcinoma is the second highest malignant disease that causes death in Indonesia with 58,256 new cases in 2018. LuminalB has more aggressive clinical behavior and is considered as having unfavorable prognosis. This study aimed to determine theclinicopathological characteristics and the relationship histological grade with breast carcinoma subtype molecular luminal B.MethodsThis is a descriptive analytic study with cross sectional design using secondary data of breast carcinoma patients registered atRSUP Mohammad Hoesin Palembang during the period of January 1st, 2014 to December 31st, 2016.ResultsFrom 903 breast carcinoma patients in Mohammad Hoesin Hospital Palembang during 2014 to 2016, 43.63% had luminal Bmolecular subtype. The highest incidence and prevalence washappened in year 2016, whichwere 4.7 and 4.4 per 10 breastcarcinoma patients, respectively. The majority of breast carcinoma of luminal B molecular subtype occurs in the group of patientsaged ≥40 years (81.7%), female (98.7%), Malay race (100%), marital status (98.8%), housewives (68.3%), histopathologicalcarcinoma of invasive non-specific type (75.9%), high grade/ grade III (63.7%), localized (60.6%), no metastases (60.0%), rightsided tumor laterality (50.8%) and with negative HER2 (65.2%). There was a significant relationship between the histopathologicalgrade and breast carcinoma luminal B molecular subtype (p=0.009).ConclusionThe incident of breast carcinoma molecular subtype luminal B at Mohammad Hoesin Hospital Palembang has increased in the lastthree years with the poor clinicopathological characteristics.
Edukasi herbal dan pengolahan minuman herbal sebagai peningkat sistem imun untuk mencegah infeksi COVID-19 Indah Solihah; Rennie Puspa Novita; Riana Sari Puspita Rasyid
INDRA: Jurnal Pengabdian kepada Masyarakat Vol. 3 No. 1 (2022): April
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/indra.v3i1.144

Abstract

The COVID-19 situation at the global and national levels is still at very high risk. A good immune system is needed to protect us from COVID-19. Eating healthy meals and increasing antioxidant consumption can help us to boost the body's immune system. Some herbs in Indonesia that are useful in boosting the immune system based on the Traditional Medicine Formulary are included red ginger, lime, cinnamon, and garlic. The Red ginger rhizome contains gingerol and shogaol, cinnamon bark contains cinnamaldehyde, lime has a high vitamin C content, and garlic bulbs contain ajoene. All of these compounds can synergistically improve the immune system. These ingredients are widely available and are processed into antioxidant herbal drinks that increase the immune system. The activity was carried out using the lecture method, discussion, and pretest-posttest to measure the level of participant knowledge. After the implementation of the activity, public knowledge increased regarding herbal ingredients, their processing, and preparation. As many as 100 percent of respondents were recognized the types of immune system enhancing herbs, 72 percent were understood how to process herbs, and 92 percent were understood how to prepare these herbs.
HUBUNGAN KEBIASAAN OLAHRAGA DENGAN KADAR ANTIBODI IMMUNOGLOBULIN G SETELAH VAKSINASI COVID 19 Veny Larasati; Rohan Sabloak; Riana Sari Puspita Rasyid; Eka Febri Zulissetiana; Susilawati Susilawati; Soilia Fertilita
JURNAL KEDOKTERAN DAN KESEHATAN Vol 10, No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/JKK.V10I1.19620

Abstract

Kebiasaan olahraga identik dengan konsep FITT (Frequency, Intensity, Type, Time) untuk menentukan efektifitas dari olahraga yang dilakukan. Sistem imun tubuh responsif terhadap kebiasaan berolahraga sehingga mampu meningkatkan respon imun terhadap antigen penyusun yang terdapat di dalam vaksin. Tujuan dari penelitian ini yaitu mengetahui hubungan antara kebiasaan olahraga dengan respon imun pasca vaksinasi COVID-19 di Kota Palembang. Penelitian ini menggunakan pendekatan cross sectional terhadap individu yang mendapatkan inactivated vaccine COVID-19 dosis kedua, dengan pengambilan sampel secara consecutive sampling dengan 78 sampel. Data pada penelitian diambil dengan wawancara mengenai kebiasaan olahraga 1 bulan sebelum vaksinasi, serta dilakukan pengambilan sampel darah vena sebanyak 5 cc pada ±28-30 hari setelah vaksinasi kedua. Darah diproses menjadi serum untuk pemeriksaan kadar IgG anti s1 RBD SARS-CoV-2 menggunakan kit produksi Abbott dengan metode CMIA. Analisis data pada penelitian ini menggunakan uji Pearson’s Chi-squared atau uji Fisher’s Exact dan/atau uji penggabungan sel sebagai alternatif. Pada penelitian ini distribusi frekuensi sampel dominan pada usia dewasa muda (56,4%), jenis kelamin perempuan (52,6%) dan kadar IgG rendah (52,6%). Interval kadar IgG pada sampel adalah 42,1 – 12971,3 AU/mL, dengan rata-rata kadar IgG 1494,95 AU/mL dan median 794,35 AU/mL. Sebaran data dominan pada kelompok dengan kebiasaan olahraga (64,1%), frekuensi olahraga kurang ideal (33,3%), jenis olahraga aerobik (59%), intensitas olahraga ringan (46,2%), dan durasi olahraga sangat lama (20,5%). Tidak diperoleh hubungan yang signifikan antara kebiasaan olahraga olahraga (frekuensi, jenis, intensitas dan durasi olahraga) dengan respon imun pasca vaksinasi (P value > 0,05). Kata kunci: Kebiasaan olahraga, latihan fisik, frekuensi, jenis, intensitas, durasi, Respon Imun, COVID-19, IgG, dan vaksin inaktif.
The Effects of Intermittent Fasting on the Size and Number of Subcutaneus Adipocytes in Obese Mouse Models Veny Larasati; Riana Sari Puspita Rasyid; Soilia Fertilita; Tri Suciati; Muhammad Farhan
Folia Medica Indonesiana Vol. 60 No. 2 (2024): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v60i2.54389

Abstract

Highlights:1. This study helps bridge the gap between systemic effects and tissue-level changes, providing a deeper understanding of how histological analysis can be used to explore the effects of intermittent fasting on adipocytes and body weight regulation.2. This study contributes to obesity management through lifestyle modification, specifically intermittent fasting, by focusing on histological changes in adipose tissue.   Abstract The accumulation of adipose tissue can have deleterious effects and lead to obesity. Intermittent fasting (IF), an approach that involves time-restricted eating, has gained popularity as an obesity treatment option because it enhances insulin sensitivity and promotes beneficial changes in glucose metabolism. This study used a time-restricted meal intake (TRM) approach to assess the effects of IF on the histological features of obese mouse models' subcutaneous inguinal adipose tissue. The investigation employed an in vivo experimental posttest-only control group design. Twenty male mice were divided into four groups: a normal control group, an obese control group, a TRM group with a high-fat diet (TRM-HF), and a TRM group with a standard diet (TRM-S). The TRM treatment was administered for fourteen days, with a fasting window from 4 p.m. to 8 a.m. The pre- and post-treatment weight analyses were conducted using the paired t-test for normally distributed data and the Wilcoxon test for non-normally distributed data (p<0.05). One-way analysis of variance (ANOVA) was employed for unpaired data on the post-treatment weight. Per field of view, there were an average of 120,500 cells (49,700–136,200) in the normal control group, 68,380±9,194 cells in the obese control group, 70,860±11,029 cells in the TRM-HF group, and 79,360±5,112 cells in the TRM-S group. The average cell sizes (μm3) were 56,730.142±19,273.257 in the normal control group, 138,934.331±27,670.558 in the obese control group, 106,827.767±20,580.501 in the TRM-HF group, and 68,689.114±8,219.727 in the TRM-S group. The number of cells in each group did not differ significantly, but there were significant variations in cell size. The mice receiving TRM treatment did not exhibit substantial body weight changes, whereas the obese control group showed a significant body weight increase. In conclusion, TRM has an effect on cell size but does not affect the quantity of adipocytes in subcutaneous inguinal fat tissue.
Co-Authors Citra Dewi Eka Febri Zulissetiana Indah Solihah Krisna Murti Larasati, Veny Muhammad Farhan Nadya Virana Putri Neti Reihan Putri Awaliah Rennie Puspa Novita Rohan Sabloak Soilia Fertilita Susilawati Susilawati Tri Suciati