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All Journal VALENSI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Chemistry Fullerene Journal of Chemistry JIPI (Jurnal IPA dan Pembelajaran IPA)
Wahab, Roswanira Abdul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Physics

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A Review on Enzymatic Response to Salt Stress and Genomic/Metagenomic Analysis of Adaptation Protein in Hypersaline Environment Oyewusi, Habeebat Adekilekun; Muhammad, Muhammad; Wahab, Roswanira Abdul; Huyop, Fahrul
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.11

Abstract

Microorganisms adapted to conditions of high salinity (low water activity) provide an understanding on how the problem of maintaining an efficient cell integrity under high osmotic stress conditions that had been tackled naturally. Almost all microbes adapting to extreme situations either by intracellularly amass inorganic ions (K+) to counterbalance high salt concentration or by synthesizing and accumulating certain organic solutes called compatible solutes that confer protection without affecting cell functions and this process may be chloride ion dependent in some microorganisms. However, the use of culture-independent method like genomic or metagenomics shields more light on the microbial diversity, gene structure and regulation as well as discovery of novel genes that led to understanding of their adaptation mechanism and roles in extreme environments. Therefore, microbes that survive this natural attenuation aimed at acclimatizing with the extreme environments could serve as the sources of biotechnologically essential molecules with an extensive array of uses.
Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS) Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.34316

Abstract

Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.
Isolation and Identification of Indigenous Polypropylene-Degrading Bacteria Isolated From Bestari Landfill of Probolinggo Yuliana, Shinta; Wijayanti, Chandra; Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Fullerene Vol 10 No 2 (2025): Fullerene Journal Of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v10i2.718

Abstract

The Bestari Landfill of Probolinggo City collects a variety of plastics waste, most of it already in a degraded state. It is estimated to be potential habitat for polypropylene-degrading bacteria. Previous NGS studies identified 2,400 species of bacteria, but did not reveal their physiological and functional characteristics. This study aims to identify PP-degrading bacteria using 16S rRNA analysis. Isolation is carried out by the Enrichment method on Mineral Salt Media (MSM) containing PP granules. The research stages included sampling, isolation, and screening of bacteria for the most effective degrading agents. Six isolates of polypropylene-degrading bacteria were discovered at the Bestari Landfill of Probolinggo City, including B1UM1, B1UM2, B1UM3, B2UM1, B2UM2, and B2UM3. After 15 days of incubation, potential isolates B1UM1, B1UM2, and B2UM1 showed the highest polymer reduction of 8.25%, 7.15%, and 6.25%. Gram staining results showed that isolate B1UM1 was coccus and a Gram-positive bacterium, while isolates B1UM2 and B2UM1 were basil and Gram-positive. Genotypic identification through 16S rRNA sequence analysis showed that isolate B1UM1 had 100.0% similarity with Staphylococcus haemolyticus, BIUM2 had a 100% similarity to Bacillus cereus, and B2UM1 had a 99% had a similarity to Bacillus sp.
Taguchi-Assisted of Ligninase Production by Phanerochaete chrysosporium ITB Isolate Biofilm Mutmainah, Siti; Wahab, Roswanira Abdul; Sanjaya, Eli Hendrik; Susanti, Evi
Indonesian Journal of Chemistry Vol 25, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.103401

Abstract

The Phanerochaete chrysosporium ITB isolate can form a biofilm when grown on a support material, in which the attached cells are in a sessile state, which allows the cells to thrive better compared to other conditions. This study uses the Taguchi model to explore the ligninase (LiP, MnP, and laccase) production by P. chrysosporium ITB isolates immobilized on polypropylene (PP). This research was carried out in a laboratory experimental manner, which consisted of stages that included the preparation of spore suspension and optimization of conditions for the production of ligninase using Taguchi. The results showed that lignin peroxidase (LiP) and laccase (420.1 and 78.69 U/mg) enzymes were optimally produced using an inoculum size of 5 × 103 spore/mL, with a growth duration of 6 d at pH 3 and required 0.4 g/mL of PP to yield a 0.36% biofilm weight. The optimal production of MnP (410.23 U/mg) warranted an inoculum size of 5 × 102 spore/mL with incubation of 4 d at pH 5, and immobilization on 0.20 g/mL PP, to give a final biofilm weight of 0.09%. These results indicate a potential LiP and laccase production by the biofilm of P. chrysosporium on the polypropylene as supporting materials.
Phytochemical and Antioxidant Activity of Lemongrass Stem Extract and Cream Analysis by Fourier Transform Infrared Suaniti*, Ni Made; Riasa, I Nyoman Putu; Puspawati, Ni Made; Samirana, Oka; Wahab, Roswanira Abdul; Valineli, Samanthi Elani; Aryasuadnyana, I Nyoman Krisnanda
Jurnal IPA & Pembelajaran IPA Vol 9, No 3 (2025): SEPTEMBER 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v9i3.48054

Abstract

Lemongrass is one of the plants that grows in Indonesia, is easy to find, and contains active compounds with potential antioxidant properties. The aim of this study was to analyze phytochemical and antioxidant activity of lemongrass stem extract and functional groups in its cream formulation. The lemongrass stems were extracted using the maceration method with 96% ethanol as the solvent, and the resulting extract was evaporated to obtain a thick lemongrass stem extract. The method of analyze the lemongrass stem extract and the cream formulation was carried out using phytochemical screening and antioxidant activity tests were performed on the lemongrass stem extract against 2,2-diphenyl-1-picrylhydrazyl as a free radical and measured at a wavelength of 517 nm using a UV-Vis spectrophotometer. The cream base, and the cream containing the lemongrass stem extract ware tested by fourier transform infrared spectrophotometer to identify functional groups in the extract and cream was conducted using the KBr pellet method. The results showed that the cream with 10% lemongrass stem extract had the strongest antioxidant activity compared to the 7.5 and 5% concentrations, with an IC50 value of 722.12 ppm, which is categorized as weak antioxidant activity. Functional group analysis showed that the cream without the addition of lemongrass stem extract had no absorption or detectable functional groups, whereas the three creams with lemongrass stem extract showed increased transmittance, with the presumed functional groups being hydroxyl (OH), methyl (CH3), and carbonyl (C=O)
Co-Authors Aryasuadnyana, I Nyoman Krisnanda Eli Hendrik Sanjaya Evi Susanti Huyop, Fahrul Muhammad Muhammad Ni Made Puspawati Oyewusi, Habeebat Adekilekun Qodriyah, Nur Romadhona Lailatul Riasa, I Nyoman Putu Samirana, Oka Siti Mutmainah Suaniti*, Ni Made Valineli, Samanthi Elani Wijayanti, Chandra YULIANA, SHINTA