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Monitoring the Success of Hydroseeding Application Using Microbial Diversity in Some Post-Coal Mining Revegetation Areas, South Kalimantan: Monitoring the Success of Hydroseeding Application Using Microbial Diversity Fadhil Anshari, Muhammad; Achmad Rinaldo Fernandes , Adji; Setyo Leksono, Amin; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.06

Successful revegetation improves the whole ecosystem's integrity, including microbe habitat. The goal of this study is to monitor the success of hydroseeding applications in some post-coal mining revegetation areas using microbial diversity. This study was conducted by sampling soils at a reference site (HS) and three revegetation areas (Kuni, AR07, AH). Kuni represents a 12-year-old monoculture replantation area while AR07 and AH are representations of hydroseeded areas with different ages, 5.5 years and 3 months old. Soil samples were then analyzed to determine N, P, K, and CEC, and extracted using the CTAB/SDS method, resulting in the total genome DNA. The genes of 16S rRNA were amplified using a specific primer, followed by product quantification and purification. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Pre Kit for Illumina. The library was sequenced on Illumina platform, and 250 bp paired-end reads were generated. The data were then analyzed using Anova, biplot, and cluster analysis. Results showed that AR07 was the most similar site to HS based on soil chemical properties and had the highest value of all alpha diversity indices. The dominant presence of Burkholderiales in AH indicated improvement in soil chemical properties. The soil microbial diversity PCA analysis revealed that the similarity between HS and AH was high. It was indicating a better success of hydroseeding at AH with shorter revegetation time as compared to the monoculture replantation site in Kuni. Hydroseeding using local pioneer species is highly recommended for postcoal mining revegetation.

Analyzing the potential chemical substances reducing the ambient carbon dioxide from Sansevieria sp. Rahardian Wicaksono, Rizky; Setyo Leksono, Amin; Warih Marhendra, Agung Pramana; Damanhuri
Jurnal Pembangunan dan Alam Lestari Vol. 15 No. 1 (2024): Jurnal Pembangunan dan Alam Lestari
Publisher : Postgraduate School of Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2024.015.01.07

Air pollution is a global problem faced by most large cities worldwide. Air has a very important role in the lives of humans and other living creatures. Air is a natural resource whose sustainability must be protected into the future. Therefore, efforts are needed to contain CO levels in the air using Sansevieria plants. Therefore, this research aims to obtain optimal levels of CO-absorbing compounds in Sansevieria sp. as carbon monoxide gas reducers. This type of research uses experimental methods (quasi-experimental). Quantitative data collection was carried out based on the results of experiments in the field. Field experiments with a Complete Random Design (Complete Random Design) with the conditions of each experiment assumed to be homogeneous. The research results show this plant contains Aliphatic Hydrocarbons and Primary Aliphatic Alcohols compounds. Keywords: air pollution, CO gas, sansevieria

Water Quality Analysis and Water Pollution Effect from Upstream to Downstream of Brantas River - East Java Irawanto, Rony; M Afifudin, Alfin Fatwa; Aurina Putri, Anggraini; Wulan Cahyani, Nadila; Nurshafa Rahmania, Farhani; Soemarno; Setyo Leksono, Amin; Yanuwiadi, Bagyo; Retnaningdyah, Catur; Riniwati, Harsuko; Sugiyanto
Jurnal Pembangunan dan Alam Lestari Vol. 15 No. 1 (2024): Jurnal Pembangunan dan Alam Lestari
Publisher : Postgraduate School of Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2024.015.01.04

Brantas River is the largest river in East Java, flowing from the city of Batu upstream until downstream in Surabaya and Sidoarjo. This river plays a crucial role as a vital natural resource that supports the various needs of the surrounding communities. This study serves as a preliminary investigation with the primary aim of assessing the water quality in three different zones of the Brantas River: the upper, middle, and downstream sites. Additionally, this research includes a review to identify the organisms exposed to water pollution in the Brantas River. The study was conducted from November 2020 to April 2023, utilizing an exploratory observational method at 19 observation stations. The data obtained was subsequently analyzed using a descriptive approach. Various parameters were observed, including pH (a measure of acidity or alkalinity), hardness (the concentration of calcium and magnesium ions), alkalinity (the capacity of water to neutralize acids), nitrate, nitrite, fluoride, and heavy metals such as lead (Pb), copper (Cu), and iron (Fe). The research findings indicate that in several upstream areas of the Brantas River, there were elevated hardness, nitrate, lead (Pb), and iron (Fe) that exceeded the established water quality standards. In the middle section of the river, water quality was generally satisfactory, although a few locations exhibited slightly elevated levels of lead (Pb). Meanwhile, all locations downstream of the Brantas River demonstrated water quality that exceeded the prescribed standards. After a literature review, it was discovered that various biota in the Brantas River have been exposed to pollutants, including fish species and plants like Ipomea aquatica and Eichhornia crassipes. Therefore, it is necessary and urgent to improve water management and monitoring plants and biota in the Brantas River to preserve organisms in aquatic and terrestrial ecosystems. Keywords: Brantas river, pollution, water quality

Monitoring the Success of Hydroseeding Application Using Microbial Diversity in Some Post-Coal Mining Revegetation Areas, South Kalimantan: Monitoring the Success of Hydroseeding Application Using Microbial Diversity Fadhil Anshari, Muhammad; Achmad Rinaldo Fernandes , Adji; Setyo Leksono, Amin; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.06

Successful revegetation improves the whole ecosystem's integrity, including microbe habitat. The goal of this study is to monitor the success of hydroseeding applications in some post-coal mining revegetation areas using microbial diversity. This study was conducted by sampling soils at a reference site (HS) and three revegetation areas (Kuni, AR07, AH). Kuni represents a 12-year-old monoculture replantation area while AR07 and AH are representations of hydroseeded areas with different ages, 5.5 years and 3 months old. Soil samples were then analyzed to determine N, P, K, and CEC, and extracted using the CTAB/SDS method, resulting in the total genome DNA. The genes of 16S rRNA were amplified using a specific primer, followed by product quantification and purification. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Pre Kit for Illumina. The library was sequenced on Illumina platform, and 250 bp paired-end reads were generated. The data were then analyzed using Anova, biplot, and cluster analysis. Results showed that AR07 was the most similar site to HS based on soil chemical properties and had the highest value of all alpha diversity indices. The dominant presence of Burkholderiales in AH indicated improvement in soil chemical properties. The soil microbial diversity PCA analysis revealed that the similarity between HS and AH was high. It was indicating a better success of hydroseeding at AH with shorter revegetation time as compared to the monoculture replantation site in Kuni. Hydroseeding using local pioneer species is highly recommended for postcoal mining revegetation.

Coastal Waterbirds Diversity, and Structure under Land Use Changes in the North Coastal of Situbondo, East Java: Coastal Waterbirds Diversity, and Structure under Land Use Changes in the Situbondo Coast, East Java Kartika Dewi, Yuni; Retnaningdyah, Catur; Setyo Leksono, Amin; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.02

Coast is suitable waterbirds habitat, but land use change could negatively influence the waterbirds life. The northern coast of Situbondo Regency, East Java Province, showed variations in land use quality. This study aims to analyze interaction between coastal land use changes on waterbirds diversity and community structure in the coast. Waterbird density was recorded based on the point count along the transect 100 m2 in six stations, namely Kampung Blekok, Tanjung Pecinan, Dubibir, Cemara, Banyuglugur, and Baluran National Park as the reference site. Land use quality in coastal areas was determined by satellites imagery. Habitat preference was determined by neu index. The results showed Tanjung Pecinan and Kampung Blekok stations were favourable waterbirds habitat, dominated by agriculture and low industry areas. These stasions better supported waterbird life and closed characters to Baluran one, where was dominated by mangrove forest and agriculture. This reference site showed highest diversity (H’ 2.26) and evenness (E 0.75), while preference habitat showed lower diversity (H’ 1.39) and evenness (E 0.29). On the contrary, Banyuglugur showed an industrial area, we found a low density species. Baluran and Kampung Blekok were habitat of three IUCN red list species, such as Ciconia episcopus, Charadius javanicus, and Leptoptilos javanicus.

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