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ANTICANCER ACTIVITIES AND METABOLITE FINGERPRINTING OF UPLC-QToF-MS/MS METHOD FROM Chrysanthemum cinerariifolium (Trev) Listiyana, Anik; Lestari, Nia Ayu; Irawati, Santia; Indrawijaya, Yen Yen Ari; Annisa, Rahmi; Bhagawan, Weka Sidha; Mutiah, Roihatul; Ma'arif, Burhan
Journal of Islamic Pharmacy Vol 4, No 1 (2019): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v4i1.7728

Chrysanthemum cinerariifolium (C. cinerariifolium) is an empirically proven medicinal plant that has anticancer activities. This study aimed to profile metabolites, and cytotoxic activity of root, leaf, stem, and root extracts C. cinerariifolium on T47D cells and to determine the correlation of metabolite content with cytotoxic activity. The metabolite profile was carried out using UPLC-QToF-MS / MS, and cytotoxic activity was carried out using the MTT method. The results obtained in the form of a chromatogram were processed with the application Masslynk so that a metabolite profile data obtained. The data is then analyzed statistically using Principal Component Analysis (PCA). The results obtained on metabolite profiling showed that there were differences in metabolite profiles in the roots, stems, leaves, and chrysanthemums. The characteristic compounds in the flower section are D - (-) - Morphine and in the leaf part of genistein Â and N - [(5-Chloro-1,2,3-thiadiazol-4-yl) methyl] -1- (2-isopropyl-4 -methyl-1,3-thiazol-5-yl) -N-methyl ethanolamine. The major compounds in the roots, stems, and leaves are Orphenadrine with successive percentages of 9.11%, 10.16%, and 3.24%, and the major compounds in the flower section are D - (-) - Morphine with a percentage of 10, 86%. Furthermore, the results of the cytotoxic activity test showed differences in anticancer cytotoxic activity in the parts of the flower, leaf, stem, and root of C. cinerariifolium. Besides, there is a relationship between the metabolite content and anticancer cytotoxic activity of each part of C. cinerariifolium. The higher the level of orphenadrine compounds in the plant, the higher the potential for anticancer.Â Keywords: C. cinerariifolium, UPLC-QToF-MS/MS, T47D cells.

CYTOTOXIC ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF GENDARUSIN A-E COMPOUNDS ON ESTROGEN ALFA RECEPTORS (2JF9) Indrawijaya, Yen Yen Ari; Octavia, Nur Ika; Mutiah, Roihatul; Bhagawan, Weka Sidha; Ma'arif, Burhan
Journal of Islamic Pharmacy Vol 4, No 1 (2019): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v4i1.9037

Estrogen Alfa (ERÎ±) is a receptor used as the main marker to identify the presence of tumors in the breast.compounds Gendarusin A-E have anticancer activity by inhibiting the poliferation of cancer cells and inducing apoptosis. The purpose of this research are to predict the cytotoxic activity, physicochemical properties, and toxicity of the gendarusin A-E compound. The predictions of physicochemical properties were tested in compliance with the Five Lipinski Rules and the results of the ADME process (absorption, distribution, metabolism, and excretion) using the application pkCSM Online tool. Prediction of cytotoxic activity using Molegro Virtual Docker (MVD) by validating receptors and molecular docking. Cancer receptor protein used in Estrogen Alfa with PDB code 2JF9. Toxicity prediction using the Protox II Online tool. The results of this study indicate that the Gendarusin A-E compound didnâ€™t completed the Five Lipinski Rules. Gendarusin A-E compounds had activity against receptors Estrogen Alpha which is shown by the results of RMSD 2 and Gendarusin A compounds had the smallest Rerank Score of -70.9817 compared to other compounds. Gendarusin B compound had the highest LD50Â 1212 mg / kg and classified in grade 4.

CHANGES ON SERUM TROPONIN T LEVEL BEFORE AND AFTER TAKING STANDARD THERAPY MEDICATION IN HEART FAILURE PATIENTS Indrawijaya, Yen Yen Ari; Suharjono, Suharjono; Aminuddin, Muhammad; Retnowati, Endang; Rahman, Gilang Mauladi
Folia Medica Indonesiana Vol 56, No 1 (2020): March
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v56i1.18444

Patients with advanced heart failure (NYHA FC III and IV heart failure) had positive cardiac troponin levels in previous cohort studies. In heart failure, cardiac troponin T (cTnT) is a biomarker that is sensitive to myocardial damage, especially myocardial necrosis. However, there is still little information regarding changes in cTnT levels during standard therapy. This prospective observational study is aimed at evaluating changes in cTnT levels before and after the administration of standard therapy and evaluating symptom improvement before and after the administration of standard therapy in patients with severe heart failure. Measurement of cTnT levels and symptom improvement parameters before treatment was carried out on the first day of the inpatient and measurement after therapy was carried out on the last day of the inpatient. Sampling was done by consecutive sampling and found 30 patients in the inpatient room of the SMF Cardiovascular Disease, Dr. Soetomo Hospital, Surabaya during the months of May-July 2017. The results of the study obtained the average cTnT levels before therapy 33.48 + 31.88 pg/ml and the average cTnT levels after therapy 46.32 + 52.68 pg/ml. Based on the statistical difference test with the Wilcoxon sign-ranked test, there was no significant change in cTnT levels (p = 0.318). On the parameter of clinical symptom improvement, there was a significant decrease in pulse, respiratory rate, blood pressure, and mean arterial pressure before and after administration of therapy (p <0.05). There was no change in troponin T levels before and after the administration of therapy meant there was no worsening of myocardial necrosis.

EVALUASI PENGGUNAAN ANTIBIOTIK PADA PASIEN PASCABEDAH APENDISITIS AKUT DI RSUD KABUPATEN PASURUAN TAHUN 2018 (Penelitian dilakukan di Instalasi Rawat Inap RSUD Kabupaten Pasuruan) Wirda, Wirda Anggraini; Wiraningtias, Novia Beta; Inayatilah, Fidia Rizkiah; Indrawijaya, Yen Yen Ari
Pharmaceutical Journal of Indonesia Vol 6, No 1 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Apendisitis akut adalah infeksi yang terjadi di apendiks vermiformis sehingga perlu segera dilakukan apendektomi. Apendektomi termasuk operasi kategori bersih kontaminasi yang memungkinkan terjadinya Infeksi Luka Operasi (ILO). Penggunaan antibiotik pada pasien pascabedah diharapkan untuk mencegah terjadinya infeksi tersebut. Penelitian ini bertujuan untuk mengidentifikasi profil penggunaan antibiotik dan mengetahui kuantitas serta kualitas penggunaan antibiotik. Penelitian ini menggunakan studi cross sectional yang dilakukan secara retrospektif menggunakan data rekam medis pasien pascabedah apendisitis akut Rumah Sakit Umum Daerah (RSUD) Kabupaten Pasuruan periode Januari-Desember 2018. Analisis data yang dilakukan pada penelitian ini secara deskriptif. Hasil dari penelitian ini menunjukkan bahwa profil penggunaan antibiotik terapeutik tertinggi untuk monoterapi adalah Sefuroksim (25,81%) dan untuk terapi kombinasi adalah Fosfomisin + Metronidazol (8,06%) dan Seftriakson + Metronidazol (8,06%). Kuantitas penggunaan antibiotik menggunakan metode Anatomical Therapeutic Chemical/ Defined Daily Dose (ATC/DDD) diperoleh nilai DDD total 52,01 DDD/100 patient-days dengan antibiotik tertinggi Metronidazol rute parenteral 14,00 DDD/100 patient-days. Antibiotik yang masuk dalam segmen Drug Utilization (DU) 90% adalah (Metronidazol, Seftriakson, Fosfomisin, Sefuroksim, Gentamisin) dengan rute parenteral. Kualitas penggunaan antibiotik berdasarkan pedoman Infectious Disease Society of America (IDSA) tepat indikasi (11,86%), tepat dosis (52,54%), tepat interval (22,03%), tepat lama pemberian (28,81%) dan tepat rute (96,61%).

EVALUASI PENGGUNAAN ANTIBIOTIK PADA PASIEN PASCABEDAH APENDISITIS AKUT DI RSUD KABUPATEN PASURUAN TAHUN 2018 (Penelitian dilakukan di Instalasi Rawat Inap RSUD Kabupaten Pasuruan) Wirda, Wirda Anggraini; Wiraningtias, Novia Beta; Inayatilah, Fidia Rizkiah; Indrawijaya, Yen Yen Ari
Pharmaceutical Journal of Indonesia Vol 6, No 1 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2020.006.01.3

Apendisitis akut adalah infeksi yang terjadi di apendiks vermiformis sehingga perlu segera dilakukan apendektomi. Apendektomi termasuk operasi kategori bersih kontaminasi yang memungkinkan terjadinya Infeksi Luka Operasi (ILO). Penggunaan antibiotik pada pasien pascabedah diharapkan untuk mencegah terjadinya infeksi tersebut. Penelitian ini bertujuan untuk mengidentifikasi profil penggunaan antibiotik dan mengetahui kuantitas serta kualitas penggunaan antibiotik. Penelitian ini menggunakan studi cross sectional yang dilakukan secara retrospektif menggunakan data rekam medis pasien pascabedah apendisitis akut Rumah Sakit Umum Daerah (RSUD) Kabupaten Pasuruan periode Januari-Desember 2018. Analisis data yang dilakukan pada penelitian ini secara deskriptif. Hasil dari penelitian ini menunjukkan bahwa profil penggunaan antibiotik terapeutik tertinggi untuk monoterapi adalah Sefuroksim (25,81%) dan untuk terapi kombinasi adalah Fosfomisin + Metronidazol (8,06%) dan Seftriakson + Metronidazol (8,06%). Kuantitas penggunaan antibiotik menggunakan metode Anatomical Therapeutic Chemical/ Defined Daily Dose (ATC/DDD) diperoleh nilai DDD total 52,01 DDD/100 patient-days dengan antibiotik tertinggi Metronidazol rute parenteral 14,00 DDD/100 patient-days. Antibiotik yang masuk dalam segmen Drug Utilization (DU) 90% adalah (Metronidazol, Seftriakson, Fosfomisin, Sefuroksim, Gentamisin) dengan rute parenteral. Kualitas penggunaan antibiotik berdasarkan pedoman Infectious Disease Society of America (IDSA) tepat indikasi (11,86%), tepat dosis (52,54%), tepat interval (22,03%), tepat lama pemberian (28,81%) dan tepat rute (96,61%).

The Effectivity of Murottal Al-Qurâ€™an Exposure to Spermatogenesis of Male Mice (Mus musculus) with stress Dianti, Meilina Ratna; Mazidah, Lilik Khoiriyatul; Ari Indrawijaya, Yen Yen; Inayatilah, Fidia Rizkiah
Journal of Islamic Medicine Vol 5, No 2 (2021): JOURNAL OF ISLAMIC MEDICINE EDISI SEPTEMBER 2021
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v5i2.9886

Introduction: Stress is the body's reaction to pressure from stressors; one of the stressors that can trigger stress is a noise stressor. Stress can disrupt the body's homeostasis system, one of the cycles of spermatogenesis. Murottal Al-Qur'an therapy listening can produce a response of relaxation and peace of mind to increase healing. Methods: The study was conducted on 25 healthy male mice aged 8-12 weeks grouped randomly into five groups. The positive control group is the normal group. The negative control group is given a 12-hour stressor. The treatment groups are given 12 hours and 1, 2, and 4 hours therapy. The treatment for each group was carried out for 21 days. Spermatogenesis of mice was observed through the number of primary spermatocytes cells and spermatid cells. It takes place during the preparation of testicular histology with Hematoxylin Eosin staining. Primary spermatocyte and spermatid counts were examined under a 40x magnification microscope. Results: Murottal Al-Qur'an therapy can affect the amount of primary spermatocyte cells (p-value = 0.000) and spermatids (p-value = 0,000) that are very significant. Conclusion: Murottal Al-Qur'an therapy can be used to increase the spermatogenesis of mice with stress.Â Keywords: Stress, Noise, Spermatogenesis, Murottal Al-Qur'an.

Metabolite Profiling of Eleutherine palmifolia (L.) Merr. By HPTLC-Densitometry and its Correlation with Anticancer Activities and In Vitro Toxicity Mutiah, Roihatul; Hadya, Chorida Muhjatul; Ma'arif Z.A, Burhan; Bhagawan, Weka Sidha; Annisa, Rahmi; Indrawijaya, Yen Yen Ari; Huwaida, Fadhila Isma; Ramadhani D.A., Ria; Susilowati, Retno; Taufik, Imam
Indonesian Journal of Pharmacy Vol 30 No 3, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is a plant that used as raw material for herbal medicines. The difference of growing location the medicinal plants affects the difference of metabolite content, It also affects the pharmacological activity and toxicity of the plant. This study aims to determine the profile of metabolites (metabolite fingerprinting), anticancer profiles, toxicity/safety profiles from several different regions in Indonesia for the purpose of authentication, efficacy, safety and quality control of these plants. Samples were sampled from 6 different locations in Indonesia, they were West Java, Central Java, East Java, East Borneo, Central Borneo, and South Borneo. Metabolite fingerprinting was determined by HPTLC-densitometry method, the profile of anticancer activity and toxicity was known by the MTT-ELISA method. The difference between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Principal Component Analysis (PCA) and Hierarchical Component Analysis (HCA). Whereas the relation between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Partial Least Square (PLS). The results of the metabolite profile analysis with PCA showed that E. palmifolia from 6 different locations gave different metabolite profiles and there were 3 metabolites that had a significant effect on cluster formation. samples are Rf 055, 059, and 044. The results of the HCA analysis showed that the 6 regions are in one cluster the same one. The analysis of PLS-DA showed that the metabolites for anticancer activity are found in Rf 059, 076, 093 and Rf 034. While the metabolites for toxicity are Rf 002, Rf 044 and Rf 059.

Efektifitas Terapi Murottal Al Qurâ€™an terhadap Stres Mencit (Mus Musculus) Dianti, Meilina Ratna; Indrawijaya, Yen Yen Ari
Journal of Islamic Pharmacy Vol 6, No 1 (2021): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v6i1.10668

Stres merupakan suatu keadaan tertekan baik secara fisik maupun secara psikologis. Suatu proses yang menilai suatu peristiwa sebagai sesuatu yg mengancam, ataupun membahayakan dan individu merespon peristiwa itu pada level fisiologis, emosional, kognitif, dan perilaku. Hormon kortisol adalah hormon stres, karena hormon ini akan diproduksi lebih banyak saat tubuh mengalami stres baik fisik maupun emosional. Terapi murottal Al-Qurâ€™an merupakan salah satu terapi non farmakologis yang dapat digunakan untuk mempercepat proses penyembuhan. Lantunan Al-Qurâ€™an secara fisik mengandung unsur suara manusia. Suara dapat menurunkan hormon stres, mengaktifkan hormon endorfin alami, meningkatkan perasaan rileks dan mengalihkan perhatian dari rasa takut, cemas, dan tegang, memperbaiki sistem kimia tubuh. Surat Ar-Rahman merupakan surat Makiyah yang mempunyai karakter ayat pendekÂ dan berulang sehingga dapat menimbulkan efek relaksasi bagi pendengar. Tujuan dari penelitian ini adalah Menganalisis efektifitas pemberian terapi Murottal Al Qurâ€™an terhadap kadar hormon kortisol pada mencit yang mengalami stres. Metode penelitian ini adalah true experimental post-test only control group design. 35 mencit dibagi menjadi 5 kelompok, Kelompok K+: tidak diberi perlakuan Â murottal QS. Ar-Rahman dan tidak diberi paparan stresor kebisingan; Kelompok Kontrol (-) diberi paparan stresor kebisingan 21 hari dan tidak diberi perlakuan murottal QS. Ar-Rahman; P1 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 1 jam/hari; P2 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 2 jam/hari; P3 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 4 jam/hari. Analisis data menggunakan Median Test didapatkan nilai p value adalah 0,032 (p 0,05) menyatakan terdapat perbedaan yang signifikan kadar kortisol pada mencit dengan kontrol negatif, kontrol positif dan yang mendapat perlakuan 1, 2, dan 3. Kesimpulan penelitian ini adalah bahwa Terapi Murottal Al Quran dapat menurunkan kadar kortisol mencit yang dipapar stres kebisingan.Kata Kunci: Stres, Kebisingan, Murottal Al Quran, QS. Ar Rahman, Hormon, KortisolÂ

Analisis Faktor Yang Mempengaruhi Ketepatan Masa Studi Mahasiswa Program Studi Farmasi UIN Maulana Malik Ibrahim Malang Hakim, Abdul; Indrawijaya, Yen Yen Ari; Yuwono, Yuwono
Journal of Islamic Pharmacy Vol 6, No 1 (2021): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v6i1.11275

The 70% punctuality of graduating students is one of the quality targets of the Faculty of Medicine and Health Sciences UIN Maulana Malik Ibrahim Malang and is one of the assessment points in the LAM-PTkes accreditation. While the punctuality of graduation from the Pharmacy department FKIK UIN Malang was 20% for the 2013 class, 17% for the 2014 batch, 14% for the 2015 batch and 66% for the 2016 batch. does not meet the FKIK quality standards. This study was conducted to identify the factors that affect the punctuality of graduation for Pharmacy students so that it can help the Pharmacy department to improve the punctuality of graduation. The design of this research is an observational study that is cross-sectional. The results of this study are that there is no significant relationship between motivation and timeliness of graduation for students of the pharmaceutical study program at UIN Malang, there is no significant relationship between interest and timeliness of graduation for students of Pharmacy department UIN Malang, there is no significant relationship between intelligence There is no significant relationship between spiritual intelligence and the punctuality of graduating pharmacy study students at UIN Malang and there is no significant relationship between the scientific field and the punctuality of graduating Pharmacy study program students at UIN Malang. In conclusion, none of the factors studied had a significant relationship with the punctuality of graduating students from the pharmacy department at UIN Maulana Malik Ibrahim Malang.Keywords: punctuality of graduation, pharmacy department

Potensi Senyawa Nitazoxanide dan Arbidol sebagai Antivirus SARS-CoV-2 terhadap Reseptor NSP5 (7BQY dan 2GZ7) dan ACE2 (3D0G dan 1R4L) Ami Fini Faqiha; Yen Yen Ari Indrawijaya; Arief Suryadinata; Muhammad Amiruddin; Roihatul Mutiah
Journal of Food and Pharmaceutical Sciences Vol 10, No 1 (2022): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.3393

Nitazoxanide merupakan obat pro-drug untuk tizoxanide. Obat tersebut diketahui memiliki aktivitas antivirus pada MERS-CoV. Arbidol memiliki aktivitas antivirus pada H3N2 HA yang memiliki kesamaan struktur dengan SARS-CoV-2. Reseptor target spesifik untuk obat-obat Anti SARS-CoV-2 saat ini adalah NSP5 (Nonstructural protein) dan ACE2 (Angiotensin-converting enzyme 2). NSP5 memediasi replikasi dan transkripsi protein-protein virus sedangkan ACE2 sebagai fasilitator transport asam amino dan reseptor tempat terikatnya SARS-CoV maupun SARS-CoV-2. Tujuan penelitian ini adalah untuk mengetahui aktivitas antivirus SARS-CoV-2 dari senyawa Nitazoxanide dan Arbidol secara in silico. Senyawa Nitazoxanide dan Arbidol dipreparasi terlebih dahulu dengan melakukan pengunduhan kode PDB ID, penyiapan struktur 2D dan 3D, penentuan minimal energy, pembuatan kode SMILES serta prediksi sifat fisikokimia dan toksisitas. Setelah dipreparasi, dilanjutkan proses penambatan molekul menggunakan aplikasi PyRx-Vina®. Hasil docking dianalisis dengan perangkat lunak PyMOL® dan Discovery Studio Visualizer®. Hasil profil fisikokimia menunjukkan bahwa senyawa Arbidol dan Nitazoxanide telah memenuhi hukum Lima Lipinski dan masing-masing memiliki nilai LD50 sebesar 1350 mg/kg dan 340 mg/kg. Hasil penambatan senyawa uji dengan reseptor menghasilkan interaksi yaitu ikatan hidrogen, interaksi hidrofobik dan interaksi elektrostatik. Interaksi Nitazoxanide pada reseptor 2GZ7 serta Arbidol pada reseptor 3D0G dan 1R4L menunjukkan perbedaan yang signifikan masing-masing terhadap Lopinavir dan Chloroquine dengan nilai p < 0.05, sehingga senyawa tersebut diprediksi memiliki potensi antivirus lebih baik.

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