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The detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) in developing country Wibowo, Muhammad Rizky; Harfiani, Erna; Sarmoko, Sarmoko; Nugraha, Yudhi
Pharmacy Reports Vol. 1 No. 1 (2021): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.235 KB) | DOI: 10.51511/pr.9

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has infected the human system resulting in Covid-19, and has spread rapidly worldwide. Therefore, a fast, simple, cost-effective, and accurate detecting tool is required. The standard diagnostic tool of the World Health Organization is the reverse transcription-polymerase chain reaction (RT-PCR). This method detects the presence of viral genetic material in the human body with accurate results. However, it has several limitations in terms of equipment, personnel, duration, and cost. Therefore, a fast, simple, and sensitive alternative detection, is required, one of which is the reverse transcription loop-mediated isothermal amplification (RT-LAMP) that functions under isothermal conditions. This method is battery-driven, hence, easy to move closer to the patient. Conclusively, the RT-LAMP test for SARS CoV-2 diagnosis produces comparable sensitivity to a standard RT-PCR and is more suitable for resource-poor settings, such as rural areas of developing countries.
THE RELATIONSHIP BETWEEN SPIRITUALITY AND RESILIENCE WITH LEVEL OF DEPRESSION IN MEDICAL STUDENTS Chintia, Chintia; Nugraha, Yudhi; Muktamiroh, Hikmah; Pasiak, Taufiq Fredrik
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 12, No 3 (2023): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.72848

Abstract

Background: Depression is a mood disorder that causes distress and affects feelings, thought, and daily activities. Medical students belong to a group that is prone to depression. The high incidence of depression in medical students is caused by stressors in study loads and busy schedules, especially during the COVID-19 pandemic. COVID-19 affects mental health, including a significant increase in depression rates because everyone must adapt to constantly changing situations. Therefore, medical students need the role of spirituality and resilience as protective factors from the occurrence of depression which also allows medical students to rise when faced with difficulties. This study aimed to determine the relationship between spirituality and resilience with the level of depression in medical students.Methods: This research was an observational analytic study with a cross-sectional approach. The research sample was 246 respondents by using the Daily Spiritual Experience Scale (DSES), Connor-Davidson Resilience Scale (CD-RISC), and Beck Depression Inventory (BDI-II) questionnaires.Results: From the results of this study, it was found that the prevalence of depression in respondents was 39%. The bivariate analysis using the Chi-Square test showed a relationship between spirituality with a level of depression (p=0.000) and resilience with a level of depression (p=0.000).Conclusion: The results indicated that there is a significant relationship between spirituality and resilience with a level of depression in medical students. The higher spirituality and resilience, the lower depression level and vice versa. The lower spirituality and resilience, the higher depression level.
Studi awal: Identifikasi Keragaman Gen Bakteri Saliva pada Wanita Usia Lanjut Menggunakan Teknologi 16S Ribosome Sequencing Oxford Nanopore Adhiyanto, Chris; Hendarmin, Laifa A; Harriyati, Zeti; Suwarsono, Erike A. Suwarsono A; Suri, Auliyani Andam; Nugraha, Yudhi; Gunarti, Dwirini Retno
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.46752

Abstract

The elderly are a population particularly susceptible to diseases resulting from the decline in immune system function. The mouth is one of the areas of the body that is the entry point for microorganisms into the body. It is known that the oral microbiome greatly affects oral and systemic health. The oral bacterial profile is important to understand its role in maintaining health and causing disease. Therefore, maintaining oral health is very important, especially in our study of the elderly. Utilizing advanced sequencing techniques such as Oxford Nanopore Technology (ONT) offers the opportunity to reveal bacterial diversity comprehensively. This study aims to identify the oral microbiome in a group of elderly women. Knowledge of the oral microbiota profile could help spread awareness of the benefits of maintaining good dental hygiene, especially in older adults. Methods: Seven elderly housewives had their unstimulated saliva samples taken in the morning and kept at -80°C. The phenol-chloroform technique was used to isolate the DNA, and a Qubit Fluorometer was used to measure purity and concentration. By employing ONT for 16S rRNA sequencing and Epi2Me software for analysis, taxonomic mapping of the bacterial diversity was accomplished.  Results: According to the taxonomic analysis, Streptococcus and Veillonella were the most common genera in the samples. It was common to find species like Veillonella parvula, Veillonella atypica, Streptococcus mitis, and Streptococcus rubneri. These genera contributed to the dynamics of oral biofilms by exhibiting complex ecological interactions. With advanced sequencing technologies, this work highlights the diversity of oral bacteria in elderly women in Indonesia while providing a new perspective. Notwithstanding a few limitations, these findings provide insight into the ecological dynamics of the oral microbiota, potentially leading to clinical applications in the treatment and prevention of disease.