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All Journal JURNAL PERTANIAN QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Ilmiah Pangan Halal

Suprayatmi, Mira

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Author-ID : 1228018

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Education Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Social Sciences Veterinary Other

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Articles

PEMBERDAYAAN KELOMPOK WANITA DALAM PRODUKSI OLAHAN COKLAT Suprayatmi, Mira
Qardhul Hasan: Media Pengabdian kepada Masyarakat Vol. 1 No. 1 (2015): April
Publisher : Universitas Djuanda

Seeing the potential of natural resources, human resources as well as the functional of cocoa that can be processed into chocolate. The upstream and downstream industries development is needed. The downstream industry needs policies to encourage the growth of SMEs processing cocoa production. One of the constraints of SMEs growth among the society is the lack of knowledge about chocolate including cocoa processing. Whereas processed cocoa products could actually be produced in a household scale. Therefore it is necessary for training and mentoring program to develop the chocolate business groups in the community. Through the science and technology program, a development program was conducted to two housewives group (PKK) consist of 10 people in RW 07 Kedung Badak, tanah Sareal Bogor. The training provided are production of processed chocolate, exclusive packaging, hampers/parcel packaging, entrepreneurial motivation, on line marketing, basic finance and administration. The achievement of targets and outcomes of the program reach almost 100 percent. The groups of women have started businesses with their own brand chocolate products, receiving orders, assist in the marketing of chocolate stores and souvenir shops, held bazaars and fairs and even some members had participated as a training assistant of processed chocolate. Some constraints are the PKK group still has not registered PIRT and halal certificate with their own brands, they still cooperate with an SMEs partner. This is due to the waiting time on procedures for obtaining the certificate. Another obstacle is the problem of capital source for expansion, including semi-automatic packaging equipment.

EKSTRAKSI FIKOSIANIN DARI SPIRULINA PLANTESIS SEBAGAI BIOPIGMEN DAN ANTIOKSIDAN Rahmawati, Siti Irma; Hidayatullah, Syarif; Suprayatmi, Mira
Jurnal Pertanian Vol. 8 No. 1 (2017): APRIL
Publisher : Universitas Djuanda

Pewarna alami lebih disukai karena tidak memiliki efek yang negatif terhadap tubuh manusia sehingga mempunyai tingkat keamanan pangan yang tinggi, selain itu juga pewarna alami mudah diuraikan. Bahan pewarna alami bisa diperoleh dari berbagai jenis sumber, salah satunya yaitu spesies alga yang merupakan tumbuhan tingkat rendah di perairan. Alga terdapat 2 jenis yaitu makroalga yang berukuran besar dan mikroalga yang berukuran kecil (renik). Spesies mikroalga yang dapat menghasilkan pewarna alami salah satunya adalah Spirulina. Jenis pewarna alami yang terkandung pada mikroalga tersebut yaitu fikosianin. Pada penelitian ini, dilakukan ekstraksi fikosianin dari Spirulina platensis dengan menggunakan tiga metode berbeda yaitu Maserasi, Ultrasound-Assisted-Extraction (UAE) dan Freezing untuk mengetahui metode mana untuk menghasilkan yield dan antioksidan tertinggi dari ekstrak yang dihasilkan. Hasil dari penelitian ini didapatkan bahwa berdasarkan nilai yield tertinggi dan kandungan fikosianin, freezing merupakan metode ekstraksi terbaik dengan kandungan fikosianin yang paling tinggi yaitu sebesar 26,53%. Sedangkan nilai aktivitas antioksidan terbesar dihasilkan oleh metode ekstraksi maserasi dengan nilai IC50 sebesar 49,59 ppm. Fikosianin yang dihasilkan dari ketiga metode ekstraksi termasuk antioksidan kuat.KATA KUNCI: ekstraksi, Spirulina plantesis, fikosianin, biopigmen, antioksidanEXTRACTION OF PHYCOCYANIN FROM SPIRULINA PLANTESIS FOR BIOPIGMENT AND ANTIOXIDANTABSTRACTRecently, biopigment known as a pigment which has no impact and easy to degradated in human body and better than artificial pigment. Biopigment can be resulted from the extraction of algae that are living in the water. Spirulina plantesis is one of algae resulting a pigment and known as phycocyanin. In this research, extraction of phycocyanin was done by three method of extractions; maceration, Ultrasound-Assisted-Extraction (UAE) and Freeze-thaw. Those extraction methods were compared to decide which method that resulted highest yield of extraction and has highest antioxidant activities. From the resulted data, the highest yield and highest phycocyanin concentration was obtained by freeze-thaw extraction method. Phycocyanin concentration from freeze-thaw ectract was 26,53% (w/w). However, the highest antioxidant activities was obtained by maceraion extraction mehod. The IC50 of extract from maceration was 49,59 ppm. Hence, all extracts from different extraction methods resulted high antioxidant activities.

FORMULASI VELVA JAGUNG MANIS DENGAN PENAMBAHAN CMC Suprayatmi, Mira; Novidahlia, Noli; Ainii, Aafiyah Nuur
Jurnal Pertanian Vol. 8 No. 2 (2017): OKTOBER
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jp.v8i2.1055

Velva merupakan produk beku yang berbahan dasar buah. Pada penelitian ini jagung digunakan dalam pembuatan velva untuk menambah nilai kergaman jagung sebagai pangan lokal. Penelitian ini bertujuan mengetahui pengaruh perbedaan perbandingan jagung manis dan air serta pengaruh konsentrasi cmc terhadap velva jagung manis, mengetahui formulasi yang mengahsilkan velva jagung manis yang paling disukai, dan mengetahui kandungan serat pangan serta kandungan gizi velva yang paling disukai. Metode penelitian menggunakan Rancngan Acak Lengkap (RAL) dengan dua faktor yaitu perbandingan jagung dan air ( 1:2 dan 2:1) dan konsentrasi cmc (0,25%, 0,5%, 0.75%). Tiap perlakuan dilakukan 2 kali ulangan. Hasil uji organoleptik menunjukkan bahwa perbandingan jagung dan air 1:2 dengan penambahan cmc 0,75% memiliki tingkat kesukaan lebih tinggi. Sifat fisika pada velva jagung manis terpilih untuk overrun 9,45%, viskositas 1,17 cP dan daya leleh 12,20 menit. Kandungan Kinia pada velva jagung manis untuk kadar air 5,47%, abu 29,70%, lemak 3,00%, protein 0,20%, karbohidrat 61,63%, serat pangan 7,62% dan nilai energi 274,32%.Kata kunci: jagung manis, velva, cmc. FORMULATION OF SWEET CORN VELVA ADDING CMC ABSTRACTVelva is a frozen product made from fruit. In experimen, maize is used in making velva to increase the value of corn as the local food. This study aims to influence the differences in the ratio of sweet corn and water as well as the effect of cmc concentration on sweet corn velva, knowing which formulation yields the most favorable sweet corn velva, and to know the most preferred fiber content and nutrient content of velvet. The research method used Rancngan Random (RAL) with two factors: corn and water ratio (1: 2 and 2: 1) and concentration of cmc (0,25%, 0,5%, 0,75%). Each treatment performed 2 replications. The results of the organoleptic test showed that the ratio of corn and water 1: 2 with the addition of 0.75% cmc had higher preferences. The physical properties of sweet corn velva were selected for 9.45% overrun, 1.17 cP viscosity and 12.20 min. Kinia content in sweet corn velva for 5.47% moisture content, 29.70% ash, 3.00% fat, 0.20% protein, 61.63% carbohydrate, 7.62% dietary fiber and 274,32 energy value %.

METODE EKSTRAKSI DNA UNTUK DETEKSI MOLEKULER Hutami, Rosy; Idzni, N; Ranasasmita, R; Suprayatmi, Mira
Jurnal Pertanian Vol. 8 No. 2 (2017): OKTOBER
Publisher : Universitas Djuanda

Dalam teknik deteksi molekuler seperti Loop-Amplification Mediated Polymorphism (LAMP) dan Polymerase Chain Reaction (PCR), pembuatan hulu asam deoksiribonukleat (DNA) sangat penting. Ekstraksi fase cair dan ekstraksi fasa padat merupakan beberapa metode ekstraksi DNA yang tersedia. Tujuan dari penelitian ini adalah untuk mengkarakterisasi metode dan produk ekstraksi DNA berdasarkan kemurnian DNA, visualisasi DNA, konsentrasi DNA, dan waktu pemrosesan metode ekstraksi DNA. Metode ekstraksi yang dievaluasi meliputi metode fenol-kloroform (Metode A) sebagai ekstraksi fasa cair dan metode Ekstraksi Surefood kit (Metode B) sebagai ekstraksi fasa padat. Hasil penelitian menunjukkan bahwa Metode A dapat dilakukan pada sampel dengan konsentrasi DNA sangat rendah berkisar antara 7,00 sampai 9,45 ng / μl dengan kemurnian yang baik (1,80-2,10). Meski tidak menunjukkan DNA isolat band pada gel agarosa 1% dan membutuhkan waktu pemrosesan ± 30 jam. Metode B memiliki performa yang baik dalam mengekstraksi sampel dengan DNA dengan konsentrasi tinggi (49,67 sampai 357,28 ng / μl) dengan kemurnian yang baik (1,93 sampai 2,07). Metode ini menunjukkan band untuk setiap sampel DNA pada gel agarose 1% dan membutuhkan waktu ±1 jam. Kedua metode tersebut dapat digunakan untuk preparasi sampel dalam analisis molekuler termasuk tujuan otentikasi halal.KATA KUNCI: fenol, kloroform, Surefood kit, LAMP DNA EXTRACTION METHOD FOR MOLECULAR DETECTIONABSTRACTIn molecular detection technique such as Loop-Amplification Mediated Polymorphism (LAMP) and Polymerase Chain Reaction (PCR), right upstream preparation of deoxyribonucleic acid (DNA) is very important. Liquid phase extraction and solid phase extraction are some of DNA extraction methods those are available. The purpose of this research was to characterizedthe method and product of DNA extraction based on DNA purity, DNA visualization, DNA concentration, and processing time of DNA extraction methods. Extraction methods evaluated included phenol-chloroform method (Method A)as liquid phase extraction and Surefood Extraction kit method (Method B) as solid phase extraction. Result showed that Method A could be performed on samples with very low DNA concentrations ranging from 7.00 to 9.45 ng/µl with a good purity (1.80 to 2.10). Although, it showed no DNA isolates bands on gel agarose 1% and need ± 30 hours processing time. Method B had a good performa in extracting sample with high concentration DNA (49.67 to 357.28 ng/µl) with a good purity (1.93 to 2.07). This method showed bands for each DNA samples on gel agarose 1% and need about ± 1 hour processing time. Both methods can be used for sample preparation in molecular analysis including halal authentication purposes.

MINUMAN FUNGSIONAL READY TO DRINK BERBAHAN BAKU REMPAH LOKAL Mardiah, Mardiah; Noor, Rafiqa; Suprayatmi, Mira; Listianti, Elisa
Jurnal Ilmiah Pangan Halal Vol. 1 No. 2 (2019): Jurnal Ilmiah Pangan Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jiph.v1i2.3104

The purpose of this study was to study the extraction process of spices, obtain selected formulations based on organoleptic tests and physical stability tests. The research methodology is divided into two stages: the first stage is determining the composition of the spices (ginger, tamarind, turmeric, cardamom, lemongrass, cinnamon and cloves), using a one factor design that is the difference in the composition of the spices with 3 levels of treatment, namely (A1), (A2) and (A3) . The second stage uses the formula selected from the first stage and then is treated with 3 concentrations of spice extract (15%, 20% dan 25%) and the concentration of additional CMC (0%, 0.05% , 0.1% ). The results stated that the selected products were A3 composition spices (7.5% Curcuma xanthorrhiza extract, 25% tamarindus indica extract, 10% turmeric extract, 15% cardamom extract, 15% cinnamon extract, 15% lemongrass extract, 12.5% clove extract) with concentrations herbs 15% and CMC concentration 0.1%. This product was chosen because it was rated the most preferred. The results of physical stability analysis of selected products are quite stable with a sedimentation height of 0.04 cm on the 16th day. The selected product has a pH of 3.44, with a curcumin value of 13.70 ppm, and a total phenol of 31 ppm and an antioxidant of 2.97 ppm.

Co-Authors Ainii, Aafiyah Nuur Heni Pujiastuti Idzni, N Listianti, Elisa Mardiah Noli Novidahlia Noor, Rafiqa Rahmawati, Siti Irma Ranasasmita, R Rosy Hutami

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