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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Al-Kifayah: Ilmu Tarbiyah dan Keguruan Manilkara: Journal of Bioscience
Susila, Susila
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Veterinary Other

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Optimizing DNA Extraction and Selecting Suitable Regions for Biodiversity Assessment: A Study on Shorea leprosula Rachmat, Henti Hendalastuti; Yulita, Kusumadewi Sri; Dwiyanti, Fifi Gus; Susilowati, Arida; Arrofaha, Nawwall; Susila, Susila; Kamal, Irsyad; Siregar, Iskandar Zulkarnaen
Jurnal Manajemen Hutan Tropika Vol. 30 No. 1 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/144

Abstract

The extraction method plays a crucial role in obtaining high-quality DNA samples, which is indispensable for various molecular biology techniques and analyses, enabling a deeper comprehension of genetic information and biological processes. The objectives of the study were: a) to optimize the chloroplast DNA extraction protocol by comparing modified CTAB methods and GeneAid for both leaf and wood samples of Shorea leprosula, a major commercial timber species, and b) to identify a suitable cpDNA region that exhibits variability and universality across taxa. Total DNA was analyzed by gel electrophoresis followed by Sanger sequencing to determine the amplification success. The results revealed that trnL intron, trnL-trnF, and trnG yielded readable sequences of the expected length (maximum 586 bp, 480 bp, and 908 bp, respectively), while the rps 16 intron failed to assemble a contig. The petL-psbE region provided long readability for reverse sequences (769 bp) but not for the forward sequence (195 bp). Higher successful DNA extraction was achieved from the leaves compared to the woods. The lower sequencing quality may be attributed to suboptimal primer design, the structural features of the regions resulting from extensive repetitive sequences, and the suboptimal condition of the extraction method in eliminating wood chemical compounds.
Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia) Susila, Susila; I Putu Gede P. Damayanto; Kusumadewi Sri Yulita; Nawwall Arrofaha
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7517.2024

Abstract

Rosewood (Dalbergia latifolia) is a hardwood plant, resistant to termite attack, its wood has beautiful patterned fibers and textures, so it is widely used for furniture in luxury categories with high economic value. According to CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), rosewood trade status is included in appendix II. In the trade of rosewood in both domestic and international markets, there are relatively many obstacles and difficulties in identifying the origin of rosewood. It is very important to establish a reference on the identity of wood based on the location of origin as an effort to prevent illegal trade, namely by using a molecular-based identification approach (DNA fingerprinting). In the isolation of rosewood DNA, special techniques are required due to its hard wood characteristics. This study aims to compare DNA isolation techniques from wood and leaves of rosewood using the CTAB (Cetyl Trimethyl Ammonium Bromide) protocol and the commercial kit protocol. The research used molecular biology-based identification methods. PCR results showed that the DNA isolation protocol using the commercial kit outperformed the CTAB protocol for both leaf and wood samples. DNA isolation from wood samples was more difficult than leaf samples. Overall, the best protocol for DNA isolation from rosewood leaves and wood was to use a commercial kit.
Nilai-Nilai Pendidikan Islam Dalam Tradisi Mandi Berarak Balimau Kasai Di Dusun Simpang Baru Desa Teluk Latak Kabupaten Bengkalis Provinsi Riau Adha, Ayuni; Yulianti , Eka; Susila, Susila
Jurnal Al-Kifayah: Ilmu Tarbiyah dan Keguruan Vol. 2 No. 2 (2023): Jurnal Al-Kifayah: Ilmu Tarbiyah dan Keguruan
Publisher : Sekolah Tinggi Agama Islam (STAI) Al- Kifayah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53398/ja.v2i2.360

Abstract

The research method used is a qualitative method with observation and interview techniques with selected informants with purposive sample. The results of the study show that bathing in the Balimau Kasai procession is a tradition that is still highly maintained by the local community to this day as a form of gratitude to Allah SWT and a sense of joy because the holy month of Ramadhan has come and as a symbol of self-purification both physically and mentally ahead of the fasting month of Ramadhan. The traditional procedure for bathing in the Balimau Kasai procession includes parades, distributing cakes and water, bathing in the Balimau Kasai parade, and eating together. Bathing is carried out by several community leaders, religious leaders, and government agencies using water that has been mixed with lime. Bathing in Balimau Kasai hamlet of Simpang Baru Teluk Latak village has social, religious and cultural values for the local community
Co-Authors Adha, Ayuni Arida Susilowati Iswanto Arrofaha, Nawwall Fifi Gus Dwiyanti I PUTU GEDE P. DAMAYANTO Irsyad Kamal ISKANDAR ZULKARNAEN SIREGAR KUSUMADEWI SRI YULITA Kusumadewi Sri Yulita Nawwall Arrofaha Rachmat, Henti Hendalastuti Yulianti , Eka