Sanda Maria Cretoiu

By Sanda M. Cretoiu, Dragos Cretoiu Anca Simionescu and Laurentiu M. Popescu

Part of the book: Sex Steroids

By Adrian Dumitru, Daniela Oana Toader, Sanda Maria Cretoiu, Dragos Cretoiu, Nicolae Suciu and Beatrice Mihaela Radu

Breast cancer is the second most common cancer in women and the fifth cause contributing to death due to the cancer condition. It is essential to deeply understand the complex cellular mechanisms leading to this disease. There are multiple connections between calcium homeostasis alterations and breast cancer in the literature, but no consensus links the mechanism to the disease prognosis. Among the cells contributing to the breast cancer are the breast telocytes, which connect through gap junctions to other cells, including cancer cells and myoepithelial cells. Multiple proteins (i.e., voltage-gated calcium channels, transient receptor potential channels, STIM and Orai proteins, ether à go-go potassium channels, calcium-activated potassium channels, calcium-activated chloride channels, muscarinic acetylcholine receptors, etc.) coupled with calcium signaling pathways undergo functional and/or expression changes associated with breast cancer development and progression, and might represent promising pharmacological targets. Unraveling the mechanisms of altered calcium homeostasis in various breast cells due to the cancer condition might contribute to personalized therapeutic approaches.

Part of the book: Calcium and Signal Transduction

By Andrei-Dennis Voichitoiu, Beatrice Mihaela Radu, Luciana Pavelescu, Dragos Cretoiu, Antonia Teona Deftu, Nicolae Suciu and Sanda Maria Cretoiu

Extracellular vesicles (EVs) represent a generic term for all the secreted vesicles, which include exosomes, microvesicles, and apoptotic bodies. EVs are key partners in the intercellular communication and play an essential role in multiple physiological and pathological conditions. EVs are shuttles for cargo molecules, such as RNA (mRNA, microRNA, and other noncoding RNAs), DNA, proteins (receptors, transcription factors, enzymes, and extracellular matrix proteins), and lipids. In pathological states, including cancer, EVs might represent either useful biomarkers or can be used for therapeutic purposes. Moreover, in cancer, it was demonstrated that EVs play an essential role in drug resistance. Here, we review the role played by EVs in the most common forms of cancer, with a special focus on ovarian and breast cancers.

Part of the book: Extracellular Vesicles and Their Importance in Human Health

By Dragos Cretoiu, Maria Giuliana Vannucchi, Yihua Bei, Mirko Manetti, Maria Simonetta Faussone-Pellegrini, Lidia Ibba-Manneschi, Junjie Xiao and Sanda Maria Cretoiu

Telocytes (TCs) represent a new type of interstitial cells, and were discovered by Prof. Popescu and his collaborators from Bucharest in 2005, and described as Interstitial Cajal-Like Cells (ICLCs). In 2010, Prof. Popescu and Prof. Faussone-Pellegrini from Florence, based on their expertise in morphology, agreed that in fact ICLCs were a brand-new entity and they renamed them telocytes. TCs are characterized by specific veil- or ribbon-like extensions called telopodes. Telopodes aid TCs in forming homo- or hetero-cellular contacts; thus, assembling three-dimensional networks that organizes the stromal and the parenchymal components of the organs. TCs can transfer information to neighbor cells ensuring a short-distance communication, and remotely by the release a wide variety of extracellular vesicles: exosomes, ectosomes, and multivesicular bodies. Here, we reviewed the evolution of the interest regarding TCs in different organs, in normal and pathological conditions. The main focus was on the role of TCs in gastrointestinal tract, urinary bladder, reproductive tract, and heart. This chapter sums up information about the possibilities that TCs are capable to behave as sensors/mediators in nervous activity, to represent mesenchymal stem cell precursors in adulthood, and to control and determine the differentiation/maturation of other cell types either during development or in postnatal life.

Part of the book: Innovations in Cell Research and Therapy

By Madalina-Gabriela Barbu, Andreea-Elena Boboc, Lidia Filip, Oana-Larisa Bugnar, Dragos Cretoiu, Nicolae Suciu, Oana Daniela Toader, Sanda Maria Cretoiu and Silviu-Cristian Voinea

Stem cells are defined as undifferentiated cells that are able to unlimitedly renew themselves within controlled conditions and to differentiate into a multitude of mature cell types. Skeletal muscle stem cells, represented predominantly by satellite cells, show a variable capability of self-renewal and myogenic differentiation. They were found to be involved not only in the growth of myofibers during neonatal and juvenile life but also in the regeneration of skeletal muscles after an injury. The microenvironment in which stem cells are nourished and maintained dormant preceding division and differentiation is known as “niche.” The niche consists of myofibers, which are believed to modulate the active/inactive state of the stem cells, extracellular matrix, neural networks, blood vessels, and a multitude of soluble molecules. It was observed that changes in the composition of the niche have an impact on the stem cell functions and hierarchy. Furthermore, it seems that its layout is variable throughout the entire life, translating into a decrease in the regenerative capacity of satellite cells in aged tissues. The scope of this chapter is to provide a detailed view of the changes that occur in the skeletal stem cell niche during life and to analyze their implications on tissue regeneration. Future studies should focus on developing new therapeutic tools for diseases involving muscle atrophy.

Part of the book: Background and Management of Muscular Atrophy

By Gaspar Bogdan Severus, Ionescu Ruxandra Florentina, Enache Robert Mihai, Dobrică Elena Codruța, Crețoiu Sanda Maria, Crețoiu Dragoș and Voinea Silviu Cristian

Extracellular vesicles (EVs) represent cell-specific carriers of bioactive cargos that can be of importance in either physiological or pathological processes. Frequently, EVs are seen as intercellular communication vehicles, but it has become more and more evident that their usefulness can vary from circulating biomarkers for an early disease diagnosis to future therapeutic carriers for slowing down the evolution of different afflictions and their ability to restore damaged tissue/organs. Here, we summarize the latest progress of EVs classification, biogenesis, and characteristics. We also briefly discuss their therapeutic potential, with emphasis on their potential application in regenerative medicine.

Part of the book: Extracellular Vesicles

By Ruxandra Florentina Ionescu, Elena Codruta Cozma, Robert Mihai Enache, Sanda Maria Cretoiu, Maria Iancu, Matei Mandea, Monica Profir, Oana Alexandra Roşu and Bogdan Severus Gaspar

Microbiomics represents a new science studying the microbiome, consisting of all the microorganisms of a given community. This new science collects data about all the members of the microbial community and quantifies the molecules responsible for the structure, function, and dynamics of the microbiome. The human microbiome plays a very important role in the healthy state and in a variety of disease states. The human microbiome knowledge has evolved during the last decades and nowadays one can consider that, in particular, the gut microbiota is seen as a significant organ holding 150 times more genes compared to the human genome. This chapter will focus on discussing the normal and modified phyla and species of the gut microbiome in a variety of conditions, providing a better understanding of host-microbiome interactions. We will highlight some new associations between intestinal dysbiosis and acute or chronic inflammatory and metabolic diseases.

Part of the book: Advances in Probiotics for Health and Nutrition

By Bogdan Severus Gaspar, Monica Profir, Oana Alexandra Rosu, Ruxandra Florentina Ionescu and Sanda Maria Cretoiu

The discovery of human microbiota shed a different perspective regarding human homeostasis and immune regulation. Gut microbiota comprises a multitude of microorganisms, its composition being host-specific and evolving throughout the lifetime, being subjected to both endogenous and exogenous factors. This subject gained significant interest after the improvement of metagenomic and metabolomic studies. The gut microbiome displays several roles, such as modulating gut permeability, digestive processes, metabolic pathways, and immune responses. Any dysregulation in the complex symbiosis mechanism between humans and the intestinal microbiome might lead to variable diseases. Environmental factors and diet play a very important role in maintaining a healthy gut microbiota. In this chapter, one aims to discuss the core microbiome of healthy subjects and how different stages of dysbiosis can play a role in the initiation and progression of pathogenic mechanisms leading to several diseases, such as gastrointestinal disorders (irritable bowel syndrome, inflammatory bowel diseases, infections or diarrhea associated with antibiotics, and colon cancer), metabolic disorders, obesity, diabetes, and allergies. We underline the importance of diet and environmental factors in modulating gut microorganism concentrations. We shed light on new possible perspectives regarding the modulation of gut microbiota for improving the health status of the host.

Part of the book: Human Physiology Annual Volume 2024

By Bogdan Severus Gaspar, Monica Profir and Sanda Maria Cretoiu

Recently, the role of the gut microbiome has become more prominent in gut-brain interactions. The microbiota-gut-brain axis homeostasis is responsible for our emotional behavior, stress response, and brain neurotransmitter balance. This bidirectional communication axis between the gut and the brain is influenced by the effect of the microbiome on the metabolic pathways of the host. Intestinal bacteria intervene directly in Trp metabolism, generating signaling molecules and specific metabolites with physiological effects on both the brain and the intestine. Trp is also metabolized under the influence of the microbiome and suffers three major pathways in the organism: The serotonin (5-HT), kynurenine, and indole pathway resulting in the production of neuro-active metabolites. This current chapter aims to cover the most recent data referring to the Trp metabolites and the microbiome-gut-brain axis in major gut disorders, such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and celiac disease. These diseases are frequently associated with mood disorders.

Part of the book: Weight Loss