Reham Yahya

Follower

Following

Public Views

Interests

Uploads

Papers by Reham Yahya

Phytofabrication of zinc oxide nanoparticles with advanced characterization and its antioxidant, anticancer, and antimicrobial activity against pathogenic microorganisms

Biomass Conversion and Biorefinery

The biofabrication of zinc oxide nanoparticles by renewable sources is thought to be ecologically... more The biofabrication of zinc oxide nanoparticles by renewable sources is thought to be ecologically acceptable, clean, and nontoxic. Willow plant (Salix tetrasperma) in the current study was used to measure its ability to create zinc oxide nanoparticles (ZnONPs). The created ZnONPs were characterized via several techniques namely ultraviolet visible (UV–Vis) spectroscopy, transmission electron microscope (TEM), scanning electron microscope (SEM), dynamic light scattering (DLS), atomic force microscopy (AFM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infra-red (FTIR) spectroscopy, and Raman spectroscopy. Disk diffusion method was applied to assess the antimicrobial activity of ZnONPs. Free radical scavenging potential of different concentrations of ZnONPs and plant extract was measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Cytotoxicity against prostate cancer cells (Pc3) via the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide)-based assay. Sharp peak was observed at 310 nm indicating the creation of ZnONPs. XRD results and Raman analysis confirmed the natural crystal structure of ZnONPs and stable zinc oxide (ZnO) structure. Good antibacterial activity of ZnONPs was observed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, and Salmonella typhimurium with inhibition zones 28.10 ± 0.17, 23.83 ± 0.29, 28.33 ± 0.58, 23.83 ± 1.04, 28.33 ± 0.58, and 33.83 ± 0.76 mm, respectively. Candida albicans was also inhibited with inhibition zone 23.67 ± 0.29 mm, while Mucor circinelloide and Aspergillus fumigatus not inhibited by ZnONPs. A promising antioxidant activity of ZnONPs was observed with IC50 8.73 µg/mL compared with the IC50 15.91 µg/mL of the plant extract. Cytotoxicity test indicated the activity of ZnONPs against Pc3.

Correction to: Green fabrication of nanocomposite doped with selenium nanoparticle–based starch and glycogen with its therapeutic activity: antimicrobial, antioxidant, and anti-inflammatory in vitro

Biomass Conversion and Biorefinery

In this article, the author name Emad Abada was incorrectly written as Emad Abda. The original ar... more

Download

Green fabrication of nanocomposite doped with selenium nanoparticle–based starch and glycogen with its therapeutic activity: antimicrobial, antioxidant, and anti-inflammatory in vitro

Biomass Conversion and Biorefinery

In situ green synthesis of Cu-doped ZnO based polymers nanocomposite with studying antimicrobial, antioxidant and anti-inflammatory activities

Applied Biological Chemistry

The use of eco-friendly methods for the synthesis of nanoparticles and its nano-composite has bec... more The use of eco-friendly methods for the synthesis of nanoparticles and its nano-composite has become a public demand nowadays to reduce the risks of chemical methods. In the current study, green synthesis of Cu-doped ZnO based polymers nan-ocomposite was performed. Various instrumental analysis including UV–vis, ATR-FTIR spectroscopy, XRD, SEM coupled with energy dispersive X-ray analysis, TEM and Thermal gravimetric were used to characterize nano-composite. Highly antibacterial activity of the synthesized nano-composite was recorded against tested microorganisms with promising efficacy against bacteria namely; Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and yeast (Candida albicans) but unfortunately not against black fungus (Mucor circinelloides) and filamentous fungi Aspergillus flavus and A. niger. Anti-inflammatory of nano-composite represented by hemolysis inhibition was obse...

Download

Molecular Docking and Efficacy of Aloe vera Gel Based on Chitosan Nanoparticles against Helicobacter pylori and Its Antioxidant and Anti-Inflammatory Activities

Polymers

The medicinal administration of Aloe vera gel has become promising in pharmaceutical and cosmetic... more The medicinal administration of Aloe vera gel has become promising in pharmaceutical and cosmetic applications particularly with the development of the nanotechnology concept. Nowadays, effective H. pylori treatment is a global problem; therefore, the development of natural products with nanopolymers such as chitosan nanoparticles (CSNPs) could represent a novel strategy for the treatment of gastric infection of H. pylori. HPLC analysis of A. vera gel indicated the presence of chlorogenic acid as the main constituent (1637.09 µg/mL) with other compounds pyrocatechol (1637.09 µg/mL), catechin (1552.92 µg/mL), naringenin (528.78 µg/mL), rutin (194.39 µg/mL), quercetin (295.25 µg/mL), and cinnamic acid (37.50 µg/mL). CSNPs and A. vera gel incorporated with CSNPs were examined via TEM, indicating mean sizes of 83.46 nm and 36.54 nm, respectively. FTIR spectra showed various and different functional groups in CSNPs, A. vera gel, and A. vera gel incorporated with CSNPs. Two strains of H. ...

Download

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

Molecules

Natural origin molecules represent reliable and excellent sources to overcome some medicinal prob... more Natural origin molecules represent reliable and excellent sources to overcome some medicinal problems. The study of anticancer, anticoagulant, and antimicrobial activities of Thevetia peruviana latex were the aim of the current research. An investigation using high-performance liquid chromatography (HPLC) revealed that the major content of the flavonoids are rutin (11.45 µg/mL), quersestin (7.15 µg/mL), naringin (5.25 µg/mL), and hisperdin (6.07 µg/mL), while phenolic had chlorogenic (12.39 µg/mL), syringenic (7.45 µg/mL), and ferulic (5.07 µg/mL) acids in latex of T. peruviana. Via 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, the experiment demonstrated that latex had a potent antioxidant activity with the IC50 43.9 µg/mL for scavenging DPPH. Hemolysis inhibition was 58.5% at 1000 µg/mL of latex compared with 91.0% at 200 µg/mL of indomethacin as positive control. Negligible anticoagulant properties of latex were reported where the recorded time was 11.9 s of prothromb...

Download

Induction of hydrolytic enzyme production and antibiosis via a culture of dual fungal species isolated from soil rich with the residues of woody plants in Saudi Arabia

BioResources

Inducing hydrolytic enzymes production and antibiosis is an attractive process for industrial app... more Inducing hydrolytic enzymes production and antibiosis is an attractive process for industrial applications. The approach can be used to repress pathogenic microorganisms. Using a dual culture of Aspergillus fumigatus and Aspergillus flavus, the activities of cellulase, polygalacturonase, chitinase, β-1,3-glucanases, protease, xylanase, and β-glucosidase were 1.78, 3.87, 2.98, 2.79, 6.91, 2.89 U, and 1.43 U∙mg-1 of protein. Meanwhile, the activities were 0.87, 2.78, 0.58, 1.69, 4.45, 2.06, and 0.89 U.mg-1 of protein for A. flavus alone and 0.98, 2.98, 0.87, 1.89, 4.98, 2.58, and 0.91 U.mg-1 of protein for A. fumigatus alone. The cellulase, polygalacturonase, and chitinase activities were studied at different temperatures; 40 °C and 50 °C, which were better temperatures than 20 °C in terms of enzymes activity for A. fumigatus and the dual culture compared to A. flavus. The highest antimicromial activity was observed using the dual fungal culture, where the inhibition zones were 3.13, ...

Download

Copper oxide nanoparticles as fungistat to inhibit mycotoxins and hydrolytic enzyme production by Fusarium incarnatum isolated from garlic biomass

BioResources

Garlic (Allium sativum L.) being infected by mycotoxigenic fungi is one of the primary factors li... more Garlic (Allium sativum L.) being infected by mycotoxigenic fungi is one of the primary factors limiting its nutritional and medical value. Therefore, there is an urgent need to repress mycotoxigenic fungi utilizing safer treatments, possibly involving nanoparticles. Fusarium incarnatum was isolated from garlic (A. sativum L.) that showed fungal contamination and their identification was confirmed using amplified and sequenced internal transcribed spacer nuclear ribosomal DNA regions, which confirmed the isolation of F. incarnatum from all cultivars. Copper oxide nanoparticles (CuONPs) at different concentrations showed inhibitory activity against F. incarnatum growth and mycotoxins, particularly at 400 ppm. The production of F. incarnatum mycotoxins, i.e., beauvericins, fusarins, moniliformin, and enniatins, was inhibited to 62.8%, 45.4%, 58.1%, and 55.0%, respectively at 400 ppm of CuONPs compared to the control. Shrinkage of the F. incarnatum cell membrane and collapsing of the ce...

Download

Anticancer, antioxidant, antiviral and antimicrobial activities of Kei Apple (Dovyalis caffra) fruit

Scientific Reports

Secondary plant metabolites remain one of the key sources of therapeutic agents despite the devel... more Secondary plant metabolites remain one of the key sources of therapeutic agents despite the development of new approaches for the discovery of medicinal drugs. In the current study, chemical analysis, and biological activities of Kei apple (Dovyalis caffra) methanolic extract were evaluated. Chemical analysis was performed using HPLC and GC–MS. Antiviral and anticancer effect were assessed using the crystal violet technique and activity against human liver cells (HepG2), respectively. Antibacterial activity was tested with the disc diffusion method. The obtained results showed that chlorogenic acid (2107.96 ± 0.07 µg/g), catechin (168 ± 0.58 µg/g), and gallic acid (15.66 ± 0.02 µg/g) were the main bioactive compounds identified by HPLC techniques. While, compounds containing furan moieties, as well as levoglucosenone, isochiapin B, dotriacontane, 7-nonynoic acid and tert-hexadecanethiol, with different biological activities were identified by GC–MS. Additionally, inhibition of 2,2-d...

Download

Effect of Thevetia peruviana Seeds Extract for Microbial Pathogens and Cancer Control

International Journal of Pharmacology, 2021

PLY-expressing but not PLY-deficient pneumococci induce cardiac injury and inflammation

(A-C) Representative Western blots showing circulating cTnI and cTnT ... more (A-C) Representative Western blots showing circulating cTnI and cTnT in murine plasma following i.v. injection of D39/PLN-A (n = 4) (1x106 CFU) (A) serotype-1, sequence type 300 and 306 (n = 5) (1x106 CFU) (B), and serotype 6B and PLY-deletion pneumococci (ΔPLY) (1x106 CFU) (C). (D) Mean±SD of circulating cTnI from 4 mice (each group) that survived at 24 h after treatment with D39, PLN-A, ST300, ST306, ΔCbpA, 6B and ΔPLY (1x106 CFU each). *p<0.05 as compared to 0 h. (E) Linear correlation between pneumococcal CFU counts and circulating cTnI levels at various time points (n = 21). (F) Circulating levels of cTnI in plasma of mice following i.v. injection of D39 (1x106 CFU) with or without liposomes (lipo). (n = 3 each group). *p<0.05 as compared to 0 h, #p<0.05 as compared to D39 group. (G) Survival curves of mice infected (i.v.) with D39,PLN-A and ΔPLY (n = 10 for D39, n = 5 for PLN-A, n = 4 for ΔPLY). (H) Circulating levels of cTnI in plasma of mice that died and those that survived within the first 30 h post-D39 infection (n = 4 each group). *p<0.05 as compared to survival group. (I) Pathological examination of murine heart sections after infection with D39, serotype-1, serotype 6B and ΔCbpA pneumococci. H&E representative images (a-h) of murine heart sections under x4 magnification (a-c) and x60 magnification (d-h). Hearts from mice infected with D39 (30 h post infection, d), serotype-1 (e), 6B (f) and ΔCbpA (g) show inflammatory cell infiltration (arrows) at x60 magnification, these are absent in a normal heart section (h). (i-j) Immunohistochemistry images showing absence of pneumococcal capsule staining in heart from mice infected with D39 (i) and serotype-1 (j), despite presence of inflammatory cell infiltrations (arrows). (k) Fresh pneumococci (D39) (arrows) were stained in parallel, as a positive control for pneumococcal capsule staining. (l-n) Representative immunohistochemistry images showing absence of active caspase-3 staining in heart section from mice infected with D39 (l), serotype-1 (m) and 6B (n) despite presence of inflammatory cell infiltrations (arrows). (o) Gut microvilli of a septic mouse showing positive active caspase-3 signal (arrows), was used as a positive control for active caspase-3 staining.</p

Reham Yahya

Uploads

Papers by Reham Yahya

Log In