Papers by HUMAIRA FATIMA mais-fa19-005
In search of happiness quotient : An empirical evidence of work life balance in the Indian health care sector
Journal of Statistics and Management Systems
Guest Editors
Journal of Statistics and Management Systems
Molecules
Oxidative stress (OS) and c-Jun N-terminal kinase (JNK) are both key indicators implicated in neu... more Oxidative stress (OS) and c-Jun N-terminal kinase (JNK) are both key indicators implicated in neuro-inflammatory signalling pathways and their respective neurodegenerative diseases. Drugs targeting these factors can be considered as suitable candidates for treatment of neuronal dysfunction and memory impairment. The present study encompasses beneficial effects of a naturally occurring triterpenoid, friedelin, against scopolamine-induced oxidative stress and neurodegenerative pathologies in mice models. The treated animals were subjected to behavioural tests i.e., Y-maze and Morris water maze (MWM) for memory dysfunction. The underlying mechanism was determined via western blotting, antioxidant enzymes and lipid profile analyses. Molecular docking studies were carried out to predict the binding modes of friedelin in the binding pocket of p-JNK protein. The results reveal that scopolamine caused oxidative stress by (1) inhibiting catalase (CAT), peroxidase enzyme (POD), superoxide dis...
Polarity-guided phytochemical extraction, polyphenolic characterization, and multimode biological evaluation of Seriphidium kurramense (Qazilb.) Y. R. Ling
Arabian Journal of Chemistry
Scientific Reports
The use of the green approach for nanoparticle synthesis yielded noticeable concern due to its ec... more The use of the green approach for nanoparticle synthesis yielded noticeable concern due to its eco-friendliness, cost-effectiveness, and reduced production of toxic chemicals. The current study was designed to formulate Zinc oxide nanoparticles (ZnO NPs) by using Fagonia cretica extracts, evaluating its phytochemical content, and different biological activities. Four different solvents; methanol (MeOH), n-Hexane (n–H), aqueous (Aq), and ethyl acetate (EA), had been utilized in the extracting method. ZnO NPs were successfully synthesized and characterized by UV–vis spectroscopy and scanning electron microscopy (SEM). The UV–vis spectra showed absorbance peaks between 350–400 nm range and SEM analysis revealed spherical morphology with particle sizes ranging from 65–80 nm. In phytochemical analysis, crude extracts exhibited the highest phytochemical content as they contain enriched secondary metabolites. n-hexane extract showed the highest phenolic contents while aqueous extracts show...
Plants
Green synthesis of metal nanoparticles is of great importance in the modern health care system. I... more Green synthesis of metal nanoparticles is of great importance in the modern health care system. In this study, zinc nanoparticles (ZnONPs) were synthesized using leaf and root extracts of Withania somnifera using four different solvents. ZnONPs were characterized by UV-vis spectrophotometer with a range between 350–400 nm. Scanning electron microscope revealed spherical morphology with an overall size of 70–90 nm and XRD pattern confirmed the crystalline structure. The total flavonoids, phenolic, and alkaloid contents were significantly greater in the crude extracts as compared to ZnONPs. The highest scavenging activity was observed in ZnONPs from n-hexane and ethyl-acetate extracts of roots with IC50 values of 27.36 µg/mL and 39.44 µg/mL, respectively. ZnONPs from methanol and aqueous extracts showed significant antibacterial activity against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis while none of the extracts were found to have significant antifungal activity....
Water
The hydrology of arid to semi-arid regions is substantially different from that in humid regions ... more The hydrology of arid to semi-arid regions is substantially different from that in humid regions due to scarce hydrological data distributions with dry climates and strong evapo-transpirative conditions. In the perspective of global water shortage, food security for all of the living beings has become a matter of great concern. Efficient use of water resources both in urban and rural environments and application of non-conventional water resources for irrigation are becoming increasingly important. In order to sustain crop production, the re-use of treated wastewater for irrigation of crops could be a good option. A pot experiment was set up to evaluate effects of different doses of di-ammonium phosphate (DAP) fertilizer on lead (Pb) and nickel (Ni) phyto-availability by two cultivars of rice irrigated with city effluent. Experiment was conducted in a completely randomized design (CRD) each with three replications. The results showed effective immobilization of Pb with applied phosp...
Polymers
The aim of the current study is extraction and isolation of bergenin from Bergenia ciliata and fa... more The aim of the current study is extraction and isolation of bergenin from Bergenia ciliata and fabrication of pH-sensitive Eudragit® L100 (EL100) polymeric nanoparticles (NP) to tackle limitations of solubility. Bergenin-loaded EL100 nanoparticles (BN-NP) were fabricated via nanoprecipitation and an experimental design was conducted for optimization. A reverse phase-high performance liquid chromatography (RP-HPLC) method was developed for the quantitation of bergenin. The optimized nanoformulation was characterized by its particle size, morphology, loading capacity, entrapment efficiency, drug–excipient interaction and crystallinity. An in vitro assay was executed to gauge the release potential of pH-sensitive nanoformulation. The mean particle size, zeta potential and polydispersity index (PDI) of the optimized nanoparticles were observed to be 86.17 ± 2.1 nm, −32.33 ± 5.53 mV and 0.30 ± 0.03, respectively. The morphological analysis confirmed the spherical nature of the nanopartic...
RSC Advances, 2022
MPs' pollution was explored in Freshwater Swat riverine ecosystem sediment, surface water, an... more MPs' pollution was explored in Freshwater Swat riverine ecosystem sediment, surface water, and fish. Schizothorax plagiostomus is found to be extremely vulnerable to MP pollution. Human activities are the prime source of microplastics in SR.
Polyphenolic profiling of <i>Ipomoea carnea</i> Jacq. by HPLC-DAD and its implications in oxidative stress and cancer
<i>Ipomoea carnea</i> Jacq<i>.</i> is an important folklore medicinal pla... more <i>Ipomoea carnea</i> Jacq<i>.</i> is an important folklore medicinal plant, assessed for its underexplored biological potential. Antioxidant, cytotoxic, antiproliferative and polyphenolic profile of whole plant was evaluated using various techniques. Maximum extract recovery (29% w/w), phenolic [13.54 ± 0.27 μg GAE/mg dry weight (DW)] and flavonoid (2.11 ± 0.10 μg QE /mg DW) content were recorded in methanol-distilled water (1:1) flower extract. HPLC-DAD analysis quantified substantial amount of six different polyphenols ranging from 0.081 to 37.95 μg/mg extract. Maximum total antioxidant and reducing potential were documented in methanol-distilled water and acetone-distilled water flower extracts (42.62 ± 0.47 and 24.38 ± 0.39 μg AAE/mg DW) respectively. Ethanol-chloroform root extract manifested highest free radical scavenging (IC<sub>50</sub> of 61.22 μg/mL) while 94.64% of the extracts showed cytotoxicity against brine shrimps. Ethanol leaf extract exhibited remarkable activity against THP-1 cell line (IC<sub>50</sub> = 8 ± 0.05 μg/mL) and protein kinases (31 mm phenotype bald zone).
Bioprospecting Dodonaea viscosa Jacq.; a traditional medicinal plant for antioxidant, cytotoxic, antidiabetic and antimicrobial potential
Arabian Journal of Chemistry, 2022
Czech Journal of Food Sciences, 2021
Current research work was performed to evaluate the antioxidant, antidiabetic, thrombolytic, and ... more Current research work was performed to evaluate the antioxidant, antidiabetic, thrombolytic, and cytotoxic potential of Bougainvillea glabra leaf extracts with different solvents. Extraction of leaves was carried out by maceration using solvents of various polarity such as n-hexane, chloroform, ethyl acetate, n-butanol, and methanol. The highest values of total phenolic and flavonoid contents were assessed in methanolic extract, such as 58.57% and 29.41%, respectively. Antioxidant potential was measured by DPPH free radical, showing 65.16% scavenging activity in methanolic extract. Similarly, the reducing power of methanolic extract was also measured. Hemolytic activity of B. glabra leaf extracts was evaluated, and maximum percentage lysis was found as 6.86% in chloroform extract. The thrombolytic activity of B. glabra leaf extracts was evaluated against human red blood cells, and the maximum percentage of clot lysis was 59.10% in chloroform extract. Maximum antidiabetic activity (1...
Polarity guided extraction, HPLC based phytochemical quantification, and multimode biological evaluation of Otostegia limbata (Benth.) Boiss
Arabian Journal of Chemistry, 2021
Materials Science and Engineering: C, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Revista Brasileira de Farmacognosia, 2020
Datura innoxia Mill., Solanaceae, an underexplored ethnomedicinal plant, has been empirically eva... more Datura innoxia Mill., Solanaceae, an underexplored ethnomedicinal plant, has been empirically evaluated in the current study for the isolation of bioactive agents. A crude extract of D. innoxia leaves was subjected to normal-phase medium pressure and vacuum liquid chromatography which led to the purification of a withanolide, isowithametelin. The chemical structure was established based on single-crystal X-ray diffraction. Isowithametelin exhibited substantial cytotoxicity against tested human cancer cell lines (breast cancer MCF-7 (ATCC® HTB-22), lung carcinoma LU-1 (human lung carcinoma established in the Department of Surgical Oncology, University of Illinois at Chicago), and prostate adenocarcinoma PC-3 (ATCC® CRL-1435)) with IC 50 values of 4.3 ± 0.93, 6.9 ± 1.3, and 0.01 ± 0.001 μg/ml, respectively. It also inhibited nitric oxide production in lipopolysaccharide-activated murine macrophage RAW 264.7 cells (IC 50 value of 18.5 ± 1.8 μg/ml) and demonstrated moderate protein kinase inhibition. This work suggests a possible anticancer role of isowithametelin; in vivo studies remain necessary to corroborate in vitro efficacy.
Natural product research, Jan 6, 2018
Ipomoea carnea Jacq. is an important folklore medicinal plant, assessed for its underexplored bio... more Ipomoea carnea Jacq. is an important folklore medicinal plant, assessed for its underexplored biological potential. Antioxidant, cytotoxic, antiproliferative and polyphenolic profile of whole plant was evaluated using various techniques. Maximum extract recovery (29% w/w), phenolic [13.54 ± 0.27 μg GAE/mg dry weight (DW)] and flavonoid (2.11 ± 0.10 μg QE /mg DW) content were recorded in methanol-distilled water (1:1) flower extract. HPLC-DAD analysis quantified substantial amount of six different polyphenols ranging from 0.081 to 37.95 μg/mg extract. Maximum total antioxidant and reducing potential were documented in methanol-distilled water and acetone-distilled water flower extracts (42.62 ± 0.47 and 24.38 ± 0.39 μg AAE/mg DW) respectively. Ethanol-chloroform root extract manifested highest free radical scavenging (IC of 61.22 μg/mL) while 94.64% of the extracts showed cytotoxicity against brine shrimps. Ethanol leaf extract exhibited remarkable activity against THP-1 cell line (I...
Journal of Exploratory Research in Pharmacology, 2017
Cannabis in both its pure and altered forms has been beneficial for human use since antiquity. 1 ... more Cannabis in both its pure and altered forms has been beneficial for human use since antiquity. 1 Members of the genus Cannabis mostly produce dioecious annual herbs. 2,3 The exact number of Cannabis species is a point of great debate, as according to different researchers there are variable number of species. 4-10 The species that are most pertinent include, Cannabis sativa, Cannabis ruderalis and Cannabis indica. However, among these, the highly polymorphic species Cannabis sativa L. is considered as the most active, based on studies targeting its morphology, anatomy, phyto-chemistry and genetics. 11 The morphological diversity of this plant is phenomenal, and it has tremendous potential as foodstuff and fuel (edible food/oil from its achene), fibre (stem) and pharmaceuticals. It also has unrivalled biochemical riches with regard to its considerable balance of active and biologically significant compounds and their potential medical uses. 12 History The history of C. sativa use dates back to over 10,000 years, supporting its recognition as one of the oldest domestic plants known to humanity. 7,13 It originated from Central Asia and is one of the oldest known psychotropic drugs. C. sativa was cultivated and consumed long before civilization; therefore, uncovering the origin of its use by humans is a difficult task. Archaeological discoveries have shown that it has been recognized and acknowledged since the Neolithic era in China, (around 4000 BC). 13 However the psychoactive potential of this plant was recognized by western medicine quite latter, with the year of 1839 seeing the first of its real description of actions. 14 China's Emperor Shen Nung wrote in his 2737 BC compendium the first description of the properties and medicinal uses of C. sativa. 14 Subsequently, it was cultivated for its fibre, fuel, seeds and medicinal purposes. 11 A distinguished surgeon in ancient China, Hua Tuo (115-205 AD), reportedly used cannabis as an anaesthetic. The analgesic and anaesthetic tendencies of C. sativa were also revealed in the biography of Chinese physician Hoa-tho, who practiced around 220 AD. 15 C. sativa then spread to the rest of the world, to ancient Egypt, prehistoric Europe, ancient Greece and
BMC Complementary and Alternative Medicine, 2015
Background: The present study aims to probe the impact of polarity dependent extraction efficienc... more Background: The present study aims to probe the impact of polarity dependent extraction efficiency variation on pharmacological spectrum of Datura innoxia Mill. in order to reconnoiter its underexplored therapeutic potential. Methods: A range of solvent extracts was subjected to phytochemical and biological assays to find the most proficient solvent system and plant part for each type of bioactivity. Total phenolic and flavonoid contents were determined colorimetrically and specific polyphenols were quantified by HPLC-DAD analysis. The samples were biologically evaluated by employing multimode antioxidant, cytotoxic, protein kinase inhibition and antimicrobial assays. Results: Among all the solvents used, maximum percent extract recovery (33.28 %) was obtained in aqueous leaf extract. The highest amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was obtained in the distilled water and ethyl acetate-ethanol extracts of leaf i.e., 29.91 ± 0.12 and 15.68 ± 0.18 mg/g dry weight (DW) respectively. Reverse phase HPLC-DAD based quantification revealed the presence of significant amounts of catechin, caffiec acid, apigenin and rutin ranging from 0.16 to 5.41 mg/g DW. Highest DPPH radical scavenging activity (IC 50 = 16.14 μg/ml) was displayed by the ethyl acetate-acetone stem extract. Maximum total antioxidant capacity and reducing power potential were recorded in the aqueous leaf and ethyl acetate stem extracts i.e., 46.98 ± 0.24 and 15.35 ± 0.61 mg ascorbic acid equivalent/g DW respectively. Cytotoxicity against brine shrimps categorized 25 % of the leaf, 16 % of the stem and 8.3 % of the fruit extracts as highly potent (LC 50 ≤ 100 μg/ml). Significant cytotoxicity against human leukemia (THP-1) cell line was exhibited by the chloroform and n-hexane fruit extracts with IC 50 4.52 and 3.49 μg/ml respectively. Ethyl acetate and methanol-chloroform extracts of leaf and stem exhibited conspicuous protein kinase inhibitory activity against Streptomyces 85E strain with 22 mm bald phenotype. A noteworthy antimicrobial activity was exhibited by leaf extracts against Micrococcus luteus and n-hexane fruit extract against Aspergillus niger (MIC 3.70 and 12.5 μg/ml respectively). Conclusion: Multiple solvent system is a crucial variable to retrieve pharmacological potential of medicinal plants and D. innoxia can be envisaged as a novel source of natural antioxidants, antimicrobials and anticancer compounds.
Journal of Applied Research on Medicinal and Aromatic Plants, 2015
The validation of ethno botanical data from the under explored folk plant remedies represent an i... more The validation of ethno botanical data from the under explored folk plant remedies represent an inexhaustible reservoir of novel compounds for drug discovery. The present work was aimed to evaluate the phytochemical analysis and various biological activities (antioxidant, antimicrobial, antileishmanial, cytotoxic, anticancer, and protein kinase inhibition) of Artemisia scoparia whole plant extracts prepared by employing an eclectic range of solvents polarities. Among 14 extraction solvents employed, maximum percent extract recovery (10%) was obtained when distilled water was employed as extraction solvent. The highest amount of gallic acid equivalent total phenolic (26.7 g GAE/mg extract) and quercetin equivalent flavonoid contents (18.7 g QE/mg extract) were quantified in ethanolic extract. Reverse phase HPLC-DAD based quantitative evaluation showed significant amount of rutin, quercetin, caffeic acid, kaempferol, apigenin and artemisinin ranging from 0.06 to 6.55 g/mg extract. Ethanolic extract also exhibited highest DPPH scavenging (45.82%) reduction potential and total antioxidant capacity (110 and 60 g ascorbic acid equivalent/mg extract, respectively). The remarkable toxicity profile against brine shrimps and leishmania (reported for the first time) was manifested by chloroform extract with LC 50 272 g/ml and 141 g/ml, respectively. Only methanolic extract exhibited a conspicuous in vitro anticancerous activity against THP1 human leukemia cell line with IC 50 6.93 g/ml. The ethanolic extract also exhibited significant inhibitory activity against Streptomyces 85E with 15 mm bald zone which provides a useful evidence of its protein kinase inhibition potential. This study provides strong evidence that multiple solvent system is very crucial to explore the comprehensive biological potential of medicinal plants and extraction of commercially important compounds.
Arabian Journal of Chemistry, 2015
Plants are rich source of therapeutic compounds that have tremendous applications in pharmaceutic... more Plants are rich source of therapeutic compounds that have tremendous applications in pharmaceutical industry. To find new sources of antimicrobial and antioxidant agents, methanol/ chloroform and aqueous extracts of 61 medicinal plants were evaluated systematically. Antimicrobial activity was assessed against six bacterial and five fungal strains, while natural antioxidants were studied using reducing power (RP), total antioxidant capacity (TAC) and 2,2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging assay. Six plants exhibited broad spectrum antibacterial activity while two exerted significant antifungal activity. Total phenolic content (TPC) of the samples varied from 20.2 to 85.6 mg/g dry weight (DW) in M/C extracts and 5.5 to 62.1 mg/g DW in aq. extracts, expressed as gallic acid equivalents (GAE). Total flavonoid content (TFC) varied from 2.9 to 44.5 mg quercitin equivalent (QE)/g DW of sample for M/C extracts and 2.4 to 37.1 mg QE/g DW for aq. extracts. The results showed that antioxidant activities of plant species varied to a great extent not only among extracts (M/C and aq.) but also between the assays used for antioxidant evaluation. Significant linear correlation (p < 0.01) of TPC with antioxidant activities suggested their contribution to antioxidant activity. Using high performance liquid chromatog
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Papers by HUMAIRA FATIMA mais-fa19-005