diff --git a/changes/unreleased/4908.KnwujH2JruNRZSrTTvvc9B.toml b/changes/unreleased/4908.KnwujH2JruNRZSrTTvvc9B.toml
new file mode 100644
index 00000000000..a8359bfc2e4
--- /dev/null
+++ b/changes/unreleased/4908.KnwujH2JruNRZSrTTvvc9B.toml
@@ -0,0 +1,5 @@
+bugfixes = "Fix ``ResourceWarning`` when passing ``pathlib.Path`` objects to methods which accept file input"
+[[pull_requests]]
+uid = "4908"
+author_uid = "harshil21"
+closes_threads = ["4907"]
diff --git a/src/telegram/_utils/files.py b/src/telegram/_utils/files.py
index 9bacce86f3c..860c1612351 100644
--- a/src/telegram/_utils/files.py
+++ b/src/telegram/_utils/files.py
@@ -145,7 +145,8 @@ def parse_file_input(  # pylint: disable=too-many-return-statements
             path = Path(file_input)
             if local_mode:
                 return path.absolute().as_uri()
-            return InputFile(path.open(mode="rb"), filename=filename, attach=attach)
+            with path.open(mode="rb") as file_handle:
+                return InputFile(file_handle, filename=filename, attach=attach)
 
         return file_input
     if isinstance(file_input, bytes):
diff --git a/tests/_utils/test_files.py b/tests/_utils/test_files.py
index 7d0b5454416..d789bdcd622 100644
--- a/tests/_utils/test_files.py
+++ b/tests/_utils/test_files.py
@@ -156,3 +156,13 @@ def test_load_file_subprocess_pipe(self):
             proc.kill()
             # This exception may be thrown if the process has finished before we had the chance
             # to kill it.
+
+    @pytest.mark.filterwarnings("error::ResourceWarning")
+    def test_parse_file_input_path_no_resource_warning(self):
+        """Test that parsing a Path input doesn't generate ResourceWarning."""
+        test_file = data_file(filename="telegram.png")
+
+        # This should not raise a ResourceWarning
+        result = telegram._utils.files.parse_file_input(test_file)
+        assert isinstance(result, InputFile)
+        assert result.filename.endswith(".png")