Majalah Obat Tradisional
Vol 24, No 3 (2019)

Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS

Roihatul Mutiah (Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang)
Weka Sidha Bhagawan (Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang)
Burhan Ma'arif (Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang)
Jauhar Maknun Septaza Rahmandika (Department of Pharmacy, Faculty of Medical Health Science, Maulana Malik Ibrahim Islamic State University of Malang)



Article Info

Publish Date
31 Dec 2019

Abstract

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. The analysis of the content of metabolites was carried out using UPLC-QTOF-MS/MS with a stationary phase column C18 (Okta Decyl Silica), mobile phase mixture of formic acid /water 0.1/99.9 (v/v), and formic acid/acetonitrile 0,1/99,9 (v/v). The results of the analysis were interpreted using software Masslynx and continued with chemometric analysis using the method Principle Component Analysis (PCA). The results showed that there were differences in the content of the metabolite compounds in E. palmifolia bulb originating from six different regions.

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Journal Info

Abbrev

TradMedJ

Publisher

Subject

Health Professions Medicine & Pharmacology Public Health

Description

raditional Medicine Journal (Majalah Obat Tradisional), or Trad. Med. J. (ISSN 1410-5918 (print) and ISSN 2406-9086 (online)), is an international scientific journal published by Faculty of Pharmacy, Universitas Gadjah Mada, three times annually. Collaborating with Indonesian Pharmacist Association, ...